BMRB Entry 19560

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19560

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Title:
N-terminal and Middle domains of human Hsp90alpha
Deposition date:
2013-10-15
Original release date:
2013-11-11
Authors:
Dyson, Jane; Park, Sung Jean
Citation:

Citation: Dyson, H. Jane; Park, Sung Jean; Martinez-Yamout, Maria. "The client protein p53 forms a molten globule-like state in the presence of Hsp90"  Nat. Struct. Biol. 18, 537-542 (2011).
PubMed: 21460846

Assembly members:

Assembly members:
Hsp90_N_domain, polymer, 221 residues, Formula weight is not available
Hsp90_M_domain, polymer, 261 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hsp90_N_domain: GHVETFAFQAEIAQLMSLII NTFYSNKEIFLRELISNSSD ALDKIRYETLTDPSKLDSGK ELHINLIPNKQDRTLTIVDT GIGMTKADLINNLGTIAKSG TKAFMEALQAGADISMIGQF GVGFYSAYLVAEKVTVITKH NDDEQYAWESSAGGSFTVRT DTGEPMGRGTKVILHLKEDQ TEYLEERRIKEIVKKHSQFI GYPITLFVEKERDKEVSDDE A
Hsp90_M_domain: KPIWTRNPDDITNEEYGEFY KSLTNDWEDHLAVKHFSVEG QLEFRALLFVPRRAPFDLFE NRKKKNNIKLYVRRVFIMDN CEELIPEYLNFIRGVVDSED LPLNISREMLQQSKILKVIR KNLVKKCLELFTELAEDKEN YKKFYEQFSKNIKLGIHEDS QNRKKLSELLRYYTSASGDE MVSLKDYCTRMKENQKHIYY ITGETKDQVANSAFVERLRK HGLEVIYMIEPIDEYCVQQL KEFEGKTLVSVTKEGLELPE D

Data sets:
Data typeCount
13C chemical shifts1181
15N chemical shifts404
1H chemical shifts404

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-terminal domain1
2Middle domain2

Entities:

Entity 1, N-terminal domain 221 residues - Formula weight is not available

1   GLYHISVALGLUTHRPHEALAPHEGLNALA
2   GLUILEALAGLNLEUMETSERLEUILEILE
3   ASNTHRPHETYRSERASNLYSGLUILEPHE
4   LEUARGGLULEUILESERASNSERSERASP
5   ALALEUASPLYSILEARGTYRGLUTHRLEU
6   THRASPPROSERLYSLEUASPSERGLYLYS
7   GLULEUHISILEASNLEUILEPROASNLYS
8   GLNASPARGTHRLEUTHRILEVALASPTHR
9   GLYILEGLYMETTHRLYSALAASPLEUILE
10   ASNASNLEUGLYTHRILEALALYSSERGLY
11   THRLYSALAPHEMETGLUALALEUGLNALA
12   GLYALAASPILESERMETILEGLYGLNPHE
13   GLYVALGLYPHETYRSERALATYRLEUVAL
14   ALAGLULYSVALTHRVALILETHRLYSHIS
15   ASNASPASPGLUGLNTYRALATRPGLUSER
16   SERALAGLYGLYSERPHETHRVALARGTHR
17   ASPTHRGLYGLUPROMETGLYARGGLYTHR
18   LYSVALILELEUHISLEULYSGLUASPGLN
19   THRGLUTYRLEUGLUGLUARGARGILELYS
20   GLUILEVALLYSLYSHISSERGLNPHEILE
21   GLYTYRPROILETHRLEUPHEVALGLULYS
22   GLUARGASPLYSGLUVALSERASPASPGLU
23   ALA

Entity 2, Middle domain 261 residues - Formula weight is not available

1   LYSPROILETRPTHRARGASNPROASPASP
2   ILETHRASNGLUGLUTYRGLYGLUPHETYR
3   LYSSERLEUTHRASNASPTRPGLUASPHIS
4   LEUALAVALLYSHISPHESERVALGLUGLY
5   GLNLEUGLUPHEARGALALEULEUPHEVAL
6   PROARGARGALAPROPHEASPLEUPHEGLU
7   ASNARGLYSLYSLYSASNASNILELYSLEU
8   TYRVALARGARGVALPHEILEMETASPASN
9   CYSGLUGLULEUILEPROGLUTYRLEUASN
10   PHEILEARGGLYVALVALASPSERGLUASP
11   LEUPROLEUASNILESERARGGLUMETLEU
12   GLNGLNSERLYSILELEULYSVALILEARG
13   LYSASNLEUVALLYSLYSCYSLEUGLULEU
14   PHETHRGLULEUALAGLUASPLYSGLUASN
15   TYRLYSLYSPHETYRGLUGLNPHESERLYS
16   ASNILELYSLEUGLYILEHISGLUASPSER
17   GLNASNARGLYSLYSLEUSERGLULEULEU
18   ARGTYRTYRTHRSERALASERGLYASPGLU
19   METVALSERLEULYSASPTYRCYSTHRARG
20   METLYSGLUASNGLNLYSHISILETYRTYR
21   ILETHRGLYGLUTHRLYSASPGLNVALALA
22   ASNSERALAPHEVALGLUARGLEUARGLYS
23   HISGLYLEUGLUVALILETYRMETILEGLU
24   PROILEASPGLUTYRCYSVALGLNGLNLEU
25   LYSGLUPHEGLUGLYLYSTHRLEUVALSER
26   VALTHRLYSGLUGLYLEUGLULEUPROGLU
27   ASP

Samples:

sample_1: Hsp90 N domain, [U-100% 13C; U-100% 15N; U-80% 2H], 0.1 – 0.3 mM; sodium phosphate 25 mM; sodium chloride 100 mM; EDTA 1 mM; DTT 4 mM

sample_2: Hsp90 M domain, [U-100% 13C; U-100% 15N; U-80% 2H], 0.1 – 0.3 mM; sodium phosphate 25 mM; sodium chloride 100 mM; EDTA 1 mM; DTT 4 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRView v5, Johnson, One Moon Scientific - data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

UXNMR, Bruker Biospin - collection

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker Avance 900 MHz

Related Database Links:

UNP P07900 P07900
PDB
DBJ BAB20777 BAB23449 BAC36610 BAC40681 BAC82487 BAB20777 BAB23449 BAC36610 BAC82487 BAE01934
EMBL CAA30255 CAA33259 CAC39453 CAD21648 CAD66568 CAA30251 CAA33259 CAC39453 CAD21648 CAD62296
GB AAA36023 AAA36992 AAA37868 AAA53068 AAA63194 AAA36992 AAA53068 AAA63194 AAC25497 AAC48718
REF NP_001012688 NP_001017963 NP_001092042 NP_001157427 NP_001182596 NP_001012688 NP_001016282 NP_001017963 NP_001092042 NP_001103255
SP A5A6K9 O02705 P07900 P07901 P30946 A5A6K9 O02705 P07900 P07901 P11501
TPG DAA17282 DAA17282
AlphaFold Q9BVQ5 Q9BVQ5 A5A6K9 O02705 P07900 P07901 P30946 A5A6K9 O02705 P07900 P07901 P11501

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks