BMRB Entry 19559

Name: Backbone resonance assignments of Bcl-2/xL
Published: 2014-02-12

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19559

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone resonance assignments of Bcl-2/xL

Deposition date:

2013-10-15

Original release date:

2014-02-12

Authors:

Schwarten, Melanie; Ma, Peixiang; Stoldt, Matthias; Willbold, Dieter

Citation:

Citation: Ma, Peixiang; Schwarten, Melanie; Schneider, Lars; Boeske, Alexandra; Henke, Nadine; Lisak, Dmitrij; Weber, Stephan; Mohrluder, Jeannine; Stoldt, Matthias; Strodel, Birgit; Methner, Axel; Hoffmann, Silke; Weiergraber, Oliver; Willbold, Dieter. "Interaction of Bcl-2 with the Autophagy-related GABAA Receptor-associated Protein (GABARAP): BIOPHYSICAL CHARACTERIZATION AND FUNCTIONAL IMPLICATIONS." J. Biol. Chem. 288, 37204-37215 (2013).
PubMed: 24240096

Assembly members:

Assembly members:
Bcl-2_xL, polymer, 167 residues, 19387.5379 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Bcl-2_xL: GMAHAGRTGYDNREIVMKYI HYKLSQRGYEWDAGDDVEEN RTEAPEGTESEVVHLTLRQA GDDFSRRYRRDFAEMSSQLH LTPFTARGRFATVVEELFRD GVNWGRIVAFFEFGGVMCVE SVNREMSPLVDNIALWMTEY LNRHLHTWIQDNGGWDAFVE LYGPSMR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	237
15N chemical shifts	126
1H chemical shifts	126

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Bcl-2/xL	1

Entities:

Entity 1, Bcl-2/xL 167 residues - 19387.5379 Da.

1

GLY

MET

ALA

HIS

ALA

GLY

ARG

THR

GLY

TYR

2

ASP

ASN

ARG

GLU

ILE

VAL

MET

LYS

TYR

ILE

3

HIS

TYR

LYS

LEU

SER

GLN

ARG

GLY

TYR

GLU

4

TRP

ASP

ALA

GLY

ASP

VAL

GLU

ASN

5

ARG

THR

GLU

ALA

PRO

GLU

GLY

THR

GLU

SER

6

GLU

VAL

HIS

LEU

THR

LEU

ARG

GLN

ALA

7

GLY

ASP

PHE

SER

ARG

TYR

ARG

8

ASP

PHE

ALA

GLU

MET

SER

GLN

LEU

HIS

9

LEU

THR

PRO

PHE

THR

ALA

ARG

GLY

ARG

PHE

10

ALA

THR

VAL

GLU

LEU

PHE

ARG

ASP

11

GLY

VAL

ASN

TRP

GLY

ARG

ILE

VAL

ALA

PHE

12

PHE

GLU

PHE

GLY

VAL

MET

CYS

VAL

GLU

13

SER

VAL

ASN

ARG

GLU

MET

SER

PRO

LEU

VAL

14

ASP

ASN

ILE

ALA

LEU

TRP

MET

THR

GLU

TYR

15

LEU

ASN

ARG

HIS

LEU

HIS

THR

TRP

ILE

GLN

16

ASP

ASN

GLY

TRP

ASP

ALA

PHE

VAL

GLU

17

LEU

TYR

GLY

PRO

SER

MET

ARG

Samples:

13C-15N_Bcl2_xL: Bcl-2/xL, [U-13C; U-15N], 0.3 mM; Tris-HCl 20.0 mM; DTT 5.0 mM

sample_conditions_1: ionic strength: 20.000 mM; pH: 7.800; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC/HMQC	13C-15N_Bcl2_xL	isotropic	sample_conditions_1
3D HNCA	13C-15N_Bcl2_xL	isotropic	sample_conditions_1
CBCACONH (H[N[co[{CA\|ca[C]}]]])	13C-15N_Bcl2_xL	isotropic	sample_conditions_1

Software:

ANALYSIS v2.3, CCPN - Assignment, Data evaluation

NMRPipe v7.9.2013.021.23.09, NIH - Conversion, Processing

VnmrJ vdifferent ones, Agilent Technologies (formerly Varian) - data recording, spectrometer operation

NMR spectrometers:

Varian VNMRS 900 MHz
Varian VNMRS 800 MHz
Varian VNMRS 600 MHz

UniProt	P10415
PDB	1G5M 1GJH 1YSW 2O21 2O22 4AQ3 4IEH 4LVT 4LXD 4MAN 5AGW 5AGX
DBJ	BAC81344
GB	AAY60777 ABS57368 AET11880 EFB24546 ELR45759
REF	XP_003734715 XP_006756883 XP_010634580
AlphaFold	Q16197