BMRB Entry 19538

Title:
SOLUTION STRUCTURE OF THE IMS DOMAIN OF THE MITOCHONDRIAL IMPORT PROTEIN TIM21 FROM S. CEREVISIAE   PubMed: 24653034
Deposition date:
2013-10-07
Original release date:
2014-04-14
Authors:
Bajaj, Rakhi; Jaremko, Lukasz; Jaremko, Mariusz; Becker, Stefan; Zweckstetter, Markus
Citation:

Citation: Jaremko, Lukasz; Jaremko, Mariusz; Giller, Karin; Becker, Stefan; Zweckstetter, Markus. "Structure of the mitochondrial translocator protein in complex with a diagnostic ligand."  Science 343, 1363-1366 (2014).

Assembly members:

Assembly members:
entity, polymer, 127 residues, 14763.120 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Experimental source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts451
15N chemical shifts129
1H chemical shifts922

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IMS DOMAIN1

Entities:

Entity 1, IMS DOMAIN 127 residues - 14763.120 Da.

1   GLYALAMETGLYSERGLYASPTHRGLNLEU
2   PHEASNARGALAVALSERMETVALGLULYS
3   ASNLYSASPILEARGSERLEULEUGLNCYS
4   ASPASPGLYILETHRGLYLYSGLUARGLEU
5   LYSALATYRGLYGLULEUILETHRASNASP
6   LYSTRPTHRARGASNARGPROILEVALSER
7   THRLYSLYSLEUASPLYSGLUGLYARGTHR
8   HISHISTYRMETARGPHEHISVALGLUSER
9   LYSLYSLYSILEALALEUVALHISLEUGLU
10   ALALYSGLUSERLYSGLNASNTYRGLNPRO
11   ASPPHEILEASNMETTYRVALASPVALPRO
12   GLYGLULYSARGTYRTYRLEUILELYSPRO
13   LYSLEUHISPROVALSERASN

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 0.3 – 0.8 mM; sodium chloride 50 mM; HEPES 20 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CYANA, Guntert, Mumenthaler and Wuthrich - peak picking, structure solution

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ GAA23416
EMBL CAA97021 CAY79794
GB AAZ22448 AHY79399 AJP38817 AJR76137 AJR76637
REF NP_011547
SP P53220
TPG DAA08130
AlphaFold P53220

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks