BMRB Entry 19531

Title:
NMR spatial structure of the trimeric mutant TM domain of VEGFR2 receptor.
Deposition date:
2013-10-02
Original release date:
2014-07-28
Authors:
Mineev, Konstantin; Arseniev, Alexander; Shulepko, Mikhail; Lyukmanova, Ekaterina; Kirpichnikov, Mikhail
Citation:

Citation: Mineev, Konstantin; Arseniev, Alexander; Shulepko, Mikhail; Lyukmanova, Ekaterina; Kirpichnikov, Mikhail. "NMR spatial structure of the trimeric mutant TM domain of VEGFR2 receptor"  To be Published ., .-..

Assembly members:

Assembly members:
VEGFR-2tm769, polymer, 37 residues, 4161.088 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: E. coli - cell free   Vector: pET20b(+)

Experimental source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: E. coli - cell free   Vector: pET20b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
VEGFR-2tm769: EKTNLEIIILEGTAVIAMFF WLLLVIILRTVKRANGG

Data sets:
Data typeCount
13C chemical shifts171
15N chemical shifts44
1H chemical shifts310

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1VEGFR-2tm769, 11
2VEGFR-2tm769, 21
3VEGFR-2tm769, 31

Entities:

Entity 1, VEGFR-2tm769, 1 37 residues - 4161.088 Da.

1   GLULYSTHRASNLEUGLUILEILEILELEU
2   GLUGLYTHRALAVALILEALAMETPHEPHE
3   TRPLEULEULEUVALILEILELEUARGTHR
4   VALLYSARGALAASNGLYGLY

Samples:

sample_1: VEGFR-2tm769 0.6 mM; VEGFR-2tm769, [U-100% 13C; U-100% 15N], 0.6 mM; DPC, [U-100% 2H], 120 mM; sodium acetate, [U-99% 2H], 20 mM; sodium azide 3 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 20 mM; pH: 4.5; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
13C,15N - filtered NOESY-HSQCsample_1isotropicsample_conditions_1

Software:

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment, data analysis

TOPSPIN v3.0, Bruker Biospin - collection

qMDD v2.1, Orekhov, Mayzel - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 19041
PDB
DBJ BAD93138
GB AAB34594 AAB88005 AAC16450 AAI31823 EAX05462
PIR I51296
REF NP_002244 XP_001086814 XP_002745866 XP_002814831 XP_003268434
SP P35968
AlphaFold P35968

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts