BMRB Entry 19524

Name: NMR solution structure of the GS-TAMAPIN MUTATION R7A.
Published: 2014-05-27

Click here to enlarge.

PDB ID: 2mel
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19524
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

NMR solution structure of the GS-TAMAPIN MUTATION R7A.

Deposition date:

2013-09-24

Original release date:

2014-05-27

Authors:

del Rio-Portilla, F.; Ramirez-Cordero, B.

Citation:

Citation: Ramirez-Cordero, Belen; Toledano, Yanis; Cano-Sanchez, Patricia; Hernandez-Lopez, Rogelio; Flores-Solis, David; Saucedo-Yanez, Alma; Chavez-Uribe, Isabel; Brieba, Luis; Del Rio-Portilla, Federico. "Cytotoxicity of recombinant tamapin and related toxin-like peptides on model cell lines." Chem. Res. Toxicol. 27, 960-967 (2014).
PubMed: 24821061

Assembly members:

Assembly members:
entity, polymer, 32 residues, 3528.202 Da.

Natural source:

Natural source: Common Name: scorpions Taxonomy ID: 34647 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mesobuthus tamulus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: modified pEt32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: SAFCNLRACELSCRSLGLLG KCIGEECKCVPY

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	215

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GS-TAMAPIN MUTATION R7A	1

Entities:

Entity 1, GS-TAMAPIN MUTATION R7A 32 residues - 3528.202 Da.

1

SER

ALA

PHE

CYS

ASN

LEU

ARG

ALA

CYS

GLU

2

LEU

SER

CYS

ARG

SER

LEU

GLY

LEU

GLY

3

LYS

CYS

ILE

GLY

GLU

CYS

LYS

CYS

VAL

4

PRO

TYR

Samples:

sample_1: entity 2.5 ± 0.1 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 3.35; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

AMBER v99, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

Varian INOVA 500 MHz