BMRB Entry 19503

Title:
Circular Permutant of the WW Domain with Loop 1 Excised
Deposition date:
2013-09-18
Original release date:
2014-01-13
Authors:
Kier, Brandon
Citation:

Citation: Kier, Brandon; Anderson, Jordan; Andersen, Niels. "Circular Permutation of a WW Domain: Folding Still Occurs after Excising the Turn of the Folding-Nucleating Hairpin."  J. Am. Chem. Soc. 136, 741-749 (2014).
PubMed: 24350581

Assembly members:

Assembly members:
entity, polymer, 29 residues, 3794.438 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: RWFYFNRITGKRQFERPKGL VKGWEKRWD

Data sets:
Data typeCount
1H chemical shifts225

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1WW Domain with Loop 1 Excised1

Entities:

Entity 1, WW Domain with Loop 1 Excised 29 residues - 3794.438 Da.

Residues 18-22 are a non-native loop that replaces and joins the N and C terminal regions of the standard-topology WW domain.

1   ARGTRPPHETYRPHEASNARGILETHRGLY
2   LYSARGGLNPHEGLUARGPROLYSGLYLEU
3   VALLYSGLYTRPGLULYSARGTRPASP

Samples:

sample_1: WW Domain with Loop 1 Excised 1.2 mM; sodium phosphate 50 mM; DSS 0.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.11 M; pH: 6.5; pressure: 1 atm; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB