BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19495

Title: HN, NH, CA, CB and methyl group assignments of Filamin C two Ig-domain fragment FLNc4-5   PubMed: 24414222

Deposition date: 2013-09-16 Original release date: 2014-02-12

Authors: Tossavainen, Helena; Permi, Perttu

Citation: Tossavainen, Helena; Seppala, Jonne; Sethi, Ritika; Pihlajamaa, Tero; Permi, Perttu. "H(N), N(H), C (), C (), and methyl group assignments of filamin multidomain fragments IgFLNc4-5 and IgFLNa3-5."  Biomol. NMR Assignments ., .-. (2014).

Assembly members:
FLNc4-5, polymer, 195 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGTVL1

Entity Sequences (FASTA):
FLNc4-5: SMAGVQKVRAWGPGLETGQV GKSADFVVEAIGTEVGTLGF SIEGPSQAKIECDDKGDGSC DVRYWPTEPGEYAVHVICDD EDIRDSPFIAHILPAPPDCF PDKVKAFGPGLEPTGCIVDK PAEFTIDARAAGKGDLKLYA QDADGCPIDIKVIPNGDGTF RCSYVPTKPIKHTIIISWGG VNVPKSPFRVNVGEG

Data sets:
Data typeCount
13C chemical shifts495
15N chemical shifts169
1H chemical shifts457

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FLNc4-51

Entities:

Entity 1, FLNc4-5 195 residues - Formula weight is not available

1   SERMETALAGLYVALGLNLYSVALARGALA
2   TRPGLYPROGLYLEUGLUTHRGLYGLNVAL
3   GLYLYSSERALAASPPHEVALVALGLUALA
4   ILEGLYTHRGLUVALGLYTHRLEUGLYPHE
5   SERILEGLUGLYPROSERGLNALALYSILE
6   GLUCYSASPASPLYSGLYASPGLYSERCYS
7   ASPVALARGTYRTRPPROTHRGLUPROGLY
8   GLUTYRALAVALHISVALILECYSASPASP
9   GLUASPILEARGASPSERPROPHEILEALA
10   HISILELEUPROALAPROPROASPCYSPHE
11   PROASPLYSVALLYSALAPHEGLYPROGLY
12   LEUGLUPROTHRGLYCYSILEVALASPLYS
13   PROALAGLUPHETHRILEASPALAARGALA
14   ALAGLYLYSGLYASPLEULYSLEUTYRALA
15   GLNASPALAASPGLYCYSPROILEASPILE
16   LYSVALILEPROASNGLYASPGLYTHRPHE
17   ARGCYSSERTYRVALPROTHRLYSPROILE
18   LYSHISTHRILEILEILESERTRPGLYGLY
19   VALASNVALPROLYSSERPROPHEARGVAL
20   ASNVALGLYGLUGLY

Samples:

sample_1: FLNc4-5, [U-13C; U-15N], 0.9 mM; sodium phosphate 50 mM; sodium chloride 100 mM; DTT 1 mM; H2O 93%; D2O 7%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
HCCmHm-TOCSYsample_1isotropicsample_conditions_1

Software:

VNMRJ, Agilent - collection

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts