BMRB Entry 19490

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19490

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Title:
Human cytosolic dNT-1 nucleotidase
Deposition date:
2013-09-11
Original release date:
2014-02-12
Authors:
Hnizda, Ales; Sklenickova, Radka; Pachl, Petr; Fabry, Milan; Tosner, Zdenek; Rezacova, Pavlina; Brynda, Jiri; Veverka, Vaclav
Citation:

Citation: Hnizda, Ale; Sklenikova, Radka; Pachl, Petr; Fabry, Milan; Toner, Zdenk; Brynda, Jii; Veverka, Vaclav. "Backbone resonance assignments of human cytosolic dNT-1 nucleotidase."  Biomol. NMR Assignments ., .-. (2013).
PubMed: 24234349

Assembly members:

Assembly members:
cdN, polymer, 205 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cdN: SNAASARSVRVLVDMDGVLA DFEAGLLRGFRRRFPEEPHV PLEQRRGFLAREQYRALRPD LADKVASVYEAPGFFLDLEP IPGALDAVREMNDLPDTQVF ICTSPLLKYHHCVGEKYRWV EQHLGPQFVERIILTRDKTV VLGDLLIDDKDTVRGQEETP SWEHILFTCCHNRHLVLPPT RRRLLSWSDNWREILDSKRG AAQRE

Data sets:
Data typeCount
13C chemical shifts565
15N chemical shifts188
1H chemical shifts188

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cdN, chain 11
2cdN, chain 21

Entities:

Entity 1, cdN, chain 1 205 residues - Formula weight is not available

1   SERASNALAALASERALAARGSERVALARG
2   VALLEUVALASPMETASPGLYVALLEUALA
3   ASPPHEGLUALAGLYLEULEUARGGLYPHE
4   ARGARGARGPHEPROGLUGLUPROHISVAL
5   PROLEUGLUGLNARGARGGLYPHELEUALA
6   ARGGLUGLNTYRARGALALEUARGPROASP
7   LEUALAASPLYSVALALASERVALTYRGLU
8   ALAPROGLYPHEPHELEUASPLEUGLUPRO
9   ILEPROGLYALALEUASPALAVALARGGLU
10   METASNASPLEUPROASPTHRGLNVALPHE
11   ILECYSTHRSERPROLEULEULYSTYRHIS
12   HISCYSVALGLYGLULYSTYRARGTRPVAL
13   GLUGLNHISLEUGLYPROGLNPHEVALGLU
14   ARGILEILELEUTHRARGASPLYSTHRVAL
15   VALLEUGLYASPLEULEUILEASPASPLYS
16   ASPTHRVALARGGLYGLNGLUGLUTHRPRO
17   SERTRPGLUHISILELEUPHETHRCYSCYS
18   HISASNARGHISLEUVALLEUPROPROTHR
19   ARGARGARGLEULEUSERTRPSERASPASN
20   TRPARGGLUILELEUASPSERLYSARGGLY
21   ALAALAGLNARGGLU

Samples:

sample_1: cdN, [U-13C; U-15N], 0.3 mM; sodium phosphate 25 mM; sodium chloride 100 mM; magnesium chloride 5 mM; beta-mercaptoethanol 5 mM; sodium azide 0.01%

sample_2: cdN, [U-100% 13C; U-100% 15N; U-80% 2H], 0.3 mM; sodium phosphate 25 mM; sodium chloride 100 mM; magnesium chloride 5 mM; beta-mercaptoethanol 5 mM; sodium azide 0.01%

sample_conditions_1: ionic strength: 175 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CACBsample_2isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAA91151
GB AAF36534 AAH17454 AAH22334 ACJ13694 ADQ32487
REF NP_055410 XP_001139013 XP_002827866 XP_003279118 XP_004041011
SP Q8TCD5
TPG DAA00070
AlphaFold Q8TCD5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks