BMRB Entry 19485

Name: Chemical Shift Assignments for SVIP (small VCP/p97-interacting protein)
Published: 2013-10-09

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19485

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical Shift Assignments for SVIP (small VCP/p97-interacting protein)

Deposition date:

2013-09-10

Original release date:

2013-10-09

Authors:

Wu, Jiawen; Peng, Dungeng; Voehler, Markus; Sanders, Charles; Li, Jun

Citation:

Citation: Wu, Jiawen; Peng, Dungeng; Voehler, Markus; Sanders, Charles; Li, Jun. "Structure and expression of a novel compact myelin protein - small VCP-interacting protein (SVIP)." Biochem. Biophys. Res. Commun. 440, 173-178 (2013).
PubMed: 24055875

Assembly members:

Assembly members:
SVIPM, polymer, 85 residues, 9475.68 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SVIPM: MGLSFPSPGESAPPTPDLEE KRAKLAEAAERRQKEAASRG ILDVQSVQEKRKKKEKIEKQ IATSGPPPEGGLRWTVSLEH HHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	280
15N chemical shifts	67
1H chemical shifts	477

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SVIP mutation	1

Entities:

Entity 1, SVIP mutation 85 residues - 9475.68 Da.

Residues 78-85 represent a non-native affinity His-tag

1

MET

GLY

LEU

SER

PHE

PRO

SER

PRO

GLY

GLU

2

SER

ALA

PRO

THR

PRO

ASP

LEU

GLU

3

LYS

ARG

ALA

LYS

LEU

ALA

GLU

ALA

GLU

4

ARG

GLN

LYS

GLU

ALA

SER

ARG

GLY

5

ILE

LEU

ASP

VAL

GLN

SER

VAL

GLN

GLU

LYS

6

ARG

LYS

GLU

LYS

ILE

GLU

LYS

GLN

7

ILE

ALA

THR

SER

GLY

PRO

GLU

GLY

8

GLY

LEU

ARG

TRP

THR

VAL

SER

LEU

GLU

HIS

9

HIS

Samples:

sample_1: SVIPM, [U-99% 13C; U-99% 15N], 0.4 – 0.6 mM; potassium phosphate 25 mM; EDTA 0.1 mM; sodium chloride 25 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 50 mM; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

GB	NM_148893 AAM88869 EAW68312 EAW68313 EAW68314 EAW68315
EMBL	CAH92580
REF	NP_001126513 NP_001248626 NP_683691 XP_001173949 XP_003254381
SP	Q5R6N0 Q8NHG7
AlphaFold	Q5R6N0 Q8NHG7