BMRB Entry 19478

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19478

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Title:
Backbone assignments for TNRC6B motif I (599-683)
Deposition date:
2013-09-06
Original release date:
2010-10-04
Authors:
Hennig, Janosch; Sattler, Michael
Citation:

Citation: Pfaff, Janina; Hennig, Janosch; Herzog, Franz; Aebersold, Ruedi; Sattler, Michael; Niessing, Dierk; Meister, Gunter. "Structural features of Argonaute-GW182 protein interactions."  Proc. Natl. Acad. Sci. U.S.A. 110, .-. (2013).
PubMed: 24043833

Assembly members:

Assembly members:
TNRC6Bm1, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-TNRC6Bm1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TNRC6Bm1: GPDSMDCQAVLQTLLSRTDL DPRVLSNTGWGQTQIKQDTV WDIEEVPRPEGKSDKGTEGW ESAATQTKNSGGWGDAPSQS NQMKSGWGEL

Data sets:
Data typeCount
13C chemical shifts139
15N chemical shifts73
1H chemical shifts73

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1TNRC6Bm11

Entities:

Entity 1, TNRC6Bm1 90 residues - Formula weight is not available

1   GLYPROASPSERMETASPCYSGLNALAVAL
2   LEUGLNTHRLEULEUSERARGTHRASPLEU
3   ASPPROARGVALLEUSERASNTHRGLYTRP
4   GLYGLNTHRGLNILELYSGLNASPTHRVAL
5   TRPASPILEGLUGLUVALPROARGPROGLU
6   GLYLYSSERASPLYSGLYTHRGLUGLYTRP
7   GLUSERALAALATHRGLNTHRLYSASNSER
8   GLYGLYTRPGLYASPALAPROSERGLNSER
9   ASNGLNMETLYSSERGLYTRPGLYGLULEU

Samples:

sample_1: TNRC6Bm1, [U-13C; U-15N], 100 – 300 uM; D2O, [U-99% 2H], 10%; H2O 90%; sodium phosphate 20 mM; sodium chloride 250 mM; sodium azide 0.02%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks