BMRB Entry 19467

Name: 10 C-terminal PKCalpha residues
Published: 2019-12-06

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19467

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

10 C-terminal PKCalpha residues

Deposition date:

2013-08-29

Original release date:

2019-12-06

Authors:

Erlendsson, Simon; Teilum, Kaare

Citation:

Citation: Erlendsson, Simon; Rathje, Mette; Heidarsson, Petur; Poulsen, Flemming; Madsen, Kenneth; Teilum, Kaare; Gether, Ulrik. "Protein interacting with C-kinase 1 (PICK1) binding promiscuity relies on unconventional PSD-95/discs-large/ZO-1 homology (PDZ) binding modes for nonclass II PDZ ligand" J. Biol. Chem. 289, 25327-25340 (2014).
PubMed: 25023278

Assembly members:

Assembly members:
PKCalpha_C-term, polymer, 12 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PKCalpha_C-term: PQFVHPILQSAV

Data sets:

assigned_chemical_shifts
- PKCalpha_C-term_CSList

Data type	Count
1H chemical shifts	74

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PKCalpha C-term	1

Entities:

Entity 1, PKCalpha C-term 12 residues - Formula weight is not available

1

PRO

GLN

PHE

VAL

HIS

PRO

ILE

LEU

GLN

SER

2

ALA

VAL

Samples:

sample_1: PKCalpha C-term 4 mM; PICK1 50 uM; D2O 10%; DTT 0.25 mM; TRIS 50 mM; Triton X 100 0.01%; sodium cloride 125 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.4; pressure: ambient atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN_analysis, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Varian INOVA 800 MHz