BMRB Entry 19466

Name: PICK1 PDZ with 10 C-terminal ASIC1a residues
Published: 2019-12-06

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19466

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

PICK1 PDZ with 10 C-terminal ASIC1a residues

Deposition date:

2013-08-29

Original release date:

2019-12-06

Authors:

Erlendsson, Simon; Teilum, Kaare

Citation:

Citation: Erlendsson, Simon; Rathje, Mette; Heidarsson, Petur; Poulsen, Flemming; Madsen, Kenneth; Teilum, Kaare; Gether, Ulrik. "Protein interacting with C-kinase 1 (PICK1) binding promiscuity relies on unconventional PSD-95/discs-large/ZO-1 homology (PDZ) binding modes for nonclass II PDZ ligand" J. Biol. Chem. 289, 25327-25340 (2014).
PubMed: 25023278

Assembly members:

Assembly members:
PICK1_PDZ, polymer, 116 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX 4T2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PICK1_PDZ: GSPGIPVPGKVTLQKDAQNL IGISIGGGAQYCPCLYIVQV FDNTPAALDGTVAAGDEITG VNGRSIKGKTKVEVAKMIQE VKGEVTIHYNKLQADPKQLE VLFQGPARGTFEDFTC

Data sets:

assigned_chemical_shifts
- PICK1_PDZ_ASIC1a_C-terminal_major_state
- PICK1_PDZ_ASIC1a_C-terminal_minor_state

Data type	Count
13C chemical shifts	335
15N chemical shifts	126
1H chemical shifts	137

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PICK1 PDZ ASIC1a C-terminal	1

Entities:

Entity 1, PICK1 PDZ ASIC1a C-terminal 116 residues - Formula weight is not available

1

GLY

SER

PRO

GLY

ILE

PRO

VAL

PRO

GLY

LYS

2

VAL

THR

LEU

GLN

LYS

ASP

ALA

GLN

ASN

LEU

3

ILE

GLY

ILE

SER

ILE

GLY

ALA

GLN

4

TYR

CYS

PRO

CYS

LEU

TYR

ILE

VAL

GLN

VAL

5

PHE

ASP

ASN

THR

PRO

ALA

LEU

ASP

GLY

6

THR

VAL

ALA

GLY

ASP

GLU

ILE

THR

GLY

7

VAL

ASN

GLY

ARG

SER

ILE

LYS

GLY

LYS

THR

8

LYS

VAL

GLU

VAL

ALA

LYS

MET

ILE

GLN

GLU

9

VAL

LYS

GLY

GLU

VAL

THR

ILE

HIS

TYR

ASN

10

LYS

LEU

GLN

ALA

ASP

PRO

LYS

GLN

LEU

GLU

11

VAL

LEU

PHE

GLN

GLY

PRO

ALA

ARG

GLY

THR

12

PHE

GLU

ASP

PHE

THR

CYS

Samples:

PICK1-ASIC: PICK1 PDZ ASIC1a C-terminal, [U-99% 13C; U-99% 15N], 900 uM; Triton X 100 0.01%; DSS 0.25 mM; DTT 2 mM; TRIS 50 mM; sodium chloride 125 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.4; pressure: ambient atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	PICK1-ASIC	isotropic	sample_conditions_1
3D HNCO_NLS	PICK1-ASIC	isotropic	sample_conditions_1
3D HNCA_NLS	PICK1-ASIC	isotropic	sample_conditions_1
3D CBCA(CO)NH_NLS	PICK1-ASIC	isotropic	sample_conditions_1
3D HN(CO)CA_NLS	PICK1-ASIC	isotropic	sample_conditions_1
3D HNCACB_NLS	PICK1-ASIC	isotropic	sample_conditions_1
3D HCCH-TOCSY_NLS	PICK1-ASIC	isotropic	sample_conditions_1
3D H(CCO)NH_NLS	PICK1-ASIC	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN_analysis, CCPN - chemical shift assignment, data analysis, peak picking

qMDD, Maxim Mayzel, Vladislav Orekhov - processing

NMR spectrometers:

Varian INOVA 800 MHz
Varian INOVA 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks