BMRB Entry 19466

Title:
PICK1 PDZ with 10 C-terminal ASIC1a residues
Deposition date:
2013-08-29
Original release date:
2019-12-06
Authors:
Erlendsson, Simon; Teilum, Kaare
Citation:

Citation: Erlendsson, Simon; Rathje, Mette; Heidarsson, Petur; Poulsen, Flemming; Madsen, Kenneth; Teilum, Kaare; Gether, Ulrik. "Protein interacting with C-kinase 1 (PICK1) binding promiscuity relies on unconventional PSD-95/discs-large/ZO-1 homology (PDZ) binding modes for nonclass II PDZ ligand"  J. Biol. Chem. 289, 25327-25340 (2014).
PubMed: 25023278

Assembly members:

Assembly members:
PICK1_PDZ, polymer, 116 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX 4T2

Data typeCount
13C chemical shifts335
15N chemical shifts126
1H chemical shifts137

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PICK1 PDZ ASIC1a C-terminal1

Entities:

Entity 1, PICK1 PDZ ASIC1a C-terminal 116 residues - Formula weight is not available

1   GLYSERPROGLYILEPROVALPROGLYLYS
2   VALTHRLEUGLNLYSASPALAGLNASNLEU
3   ILEGLYILESERILEGLYGLYGLYALAGLN
4   TYRCYSPROCYSLEUTYRILEVALGLNVAL
5   PHEASPASNTHRPROALAALALEUASPGLY
6   THRVALALAALAGLYASPGLUILETHRGLY
7   VALASNGLYARGSERILELYSGLYLYSTHR
8   LYSVALGLUVALALALYSMETILEGLNGLU
9   VALLYSGLYGLUVALTHRILEHISTYRASN
10   LYSLEUGLNALAASPPROLYSGLNLEUGLU
11   VALLEUPHEGLNGLYPROALAARGGLYTHR
12   PHEGLUASPPHETHRCYS

Samples:

PICK1-ASIC: PICK1 PDZ ASIC1a C-terminal, [U-99% 13C; U-99% 15N], 900 uM; Triton X 100 0.01%; DSS 0.25 mM; DTT 2 mM; TRIS 50 mM; sodium chloride 125 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.4; pressure: ambient atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCPICK1-ASICisotropicsample_conditions_1
3D HNCO_NLSPICK1-ASICisotropicsample_conditions_1
3D HNCA_NLSPICK1-ASICisotropicsample_conditions_1
3D CBCA(CO)NH_NLSPICK1-ASICisotropicsample_conditions_1
3D HN(CO)CA_NLSPICK1-ASICisotropicsample_conditions_1
3D HNCACB_NLSPICK1-ASICisotropicsample_conditions_1
3D HCCH-TOCSY_NLSPICK1-ASICisotropicsample_conditions_1
3D H(CCO)NH_NLSPICK1-ASICisotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN_analysis, CCPN - chemical shift assignment, data analysis, peak picking

qMDD, Maxim Mayzel, Vladislav Orekhov - processing

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks