BMRB Entry 19449

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19449
MolProbity Validation Chart

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Title:
NMR structure of a BolA-like hypothetical protein RP812 from RICKETTSIA PROWAZEKII, SEATTLE STRUCTURAL GENOMICS CENTER FOR INFECTIOUS DISEASE (SSGCID)
Deposition date:
2013-08-22
Original release date:
2013-10-08
Authors:
Chen, Yu; BARNWAL, RAVI; Yang, Fan; Varani, Gabriele
Citation:

Citation: Chen, Yu; BARNWAL, Ravi; Yang, Fan; Varani, Gabriele. "NMR structure of a BolA-like hypothetical protein RP812 from RICKETTSIA PROWAZEKII, SEATTLE STRUCTURAL GENOMICS CENTER FOR INFECTIOUS DISEASE (SSGCID)"  To be Published ., .-..

Assembly members:

Assembly members:
entity, polymer, 75 residues, 8541.002 Da.

Natural source:

Natural source:   Common Name: a-proteobacteria   Taxonomy ID: 782   Superkingdom: Bacteria   Kingdom: Proteobacteria   Genus/species: Rickettsia prowazekii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MAISAEELEKILKKSFPSSV IKITDLVGDQDHYALEISDA QFNGLSLINQHKLVKNALSE ILNKKLHSISIKTIS

Data sets:
Data typeCount
13C chemical shifts320
15N chemical shifts80
1H chemical shifts509

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BolA-like hypothetical protein RP8121

Entities:

Entity 1, BolA-like hypothetical protein RP812 75 residues - 8541.002 Da.

1   METALAILESERALAGLUGLULEUGLULYS
2   ILELEULYSLYSSERPHEPROSERSERVAL
3   ILELYSILETHRASPLEUVALGLYASPGLN
4   ASPHISTYRALALEUGLUILESERASPALA
5   GLNPHEASNGLYLEUSERLEUILEASNGLN
6   HISLYSLEUVALLYSASNALALEUSERGLU
7   ILELEUASNLYSLYSLEUHISSERILESER
8   ILELYSTHRILESER

Samples:

sample_1: BolA-like hypothetical protein RP812, [U-98% 13C; U-98% 15N], 1.5 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 100 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

CcpNMR v2.3, CCPN - chemical shift assignment, peak picking

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
EMBL CAA15238
GB ADE30378 AFE49607 AFE50451 AFE51295 AFE52133
REF NP_221162 WP_004596876
SP Q9ZCE4
AlphaFold Q9ZCE4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks