BMRB Entry 19438

Name: tgam
Published: 2014-09-02

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PDB ID: 2mcf
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19438
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

tgam

Deposition date:

2013-08-19

Original release date:

2014-09-02

Authors:

Yang, Yinshan; Montet de Guillen, Karine; Roumestand, Christian

Citation:

Citation: Yang, Yinshan; Montet de Guillen, Karine; Roumestand, Christian. "NMR structure of Tgam" .

Assembly members:

Assembly members:
Tgam, polymer, 148 residues, 16758.439 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Ecoli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Tgam: MKYDVVIIPESFHRFDKHNM EHICPPMVIGDRSYDIAMEI VNGVDRVIKASFNASVEELE GEDCDVLYRKYTLEKEGKKG IVHVKLRKITENCPPVDGNR CSVLEFERDIECIVKAIEEC LAKGELNSKLEGKPIPNPLL GLDSTRTG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	417
15N chemical shifts	136
1H chemical shifts	295

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Tgam	1

Entities:

Entity 1, Tgam 148 residues - 16758.439 Da.

1

MET

LYS

TYR

ASP

VAL

ILE

PRO

GLU

2

SER

PHE

HIS

ARG

PHE

ASP

LYS

HIS

ASN

MET

3

GLU

HIS

ILE

CYS

PRO

MET

VAL

ILE

GLY

4

ASP

ARG

SER

TYR

ASP

ILE

ALA

MET

GLU

ILE

5

VAL

ASN

GLY

VAL

ASP

ARG

VAL

ILE

LYS

ALA

6

SER

PHE

ASN

ALA

SER

VAL

GLU

LEU

GLU

7

GLY

GLU

ASP

CYS

ASP

VAL

LEU

TYR

ARG

LYS

8

TYR

THR

LEU

GLU

LYS

GLU

GLY

LYS

GLY

9

ILE

VAL

HIS

VAL

LYS

LEU

ARG

LYS

ILE

THR

10

GLU

ASN

CYS

PRO

VAL

ASP

GLY

ASN

ARG

11

CYS

SER

VAL

LEU

GLU

PHE

GLU

ARG

ASP

ILE

12

GLU

CYS

ILE

VAL

LYS

ALA

ILE

GLU

CYS

13

LEU

ALA

LYS

GLY

GLU

LEU

ASN

SER

LYS

LEU

14

GLU

GLY

LYS

PRO

ILE

PRO

ASN

PRO

LEU

15

GLY

LEU

ASP

SER

THR

ARG

THR

GLY

Samples:

sample_1: H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.2 M; pH: 6.60; pressure: 1 atm; temperature: 305 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - geometry optimization

NMR spectrometers:

Bruker Avance 700 MHz

PDB	2MCF
GB	ACS34436
REF	WP_015859542