BMRB Entry 19437

Name: Membrane induced structure of the mammalian tachykinin neuropeptide gamma
Published: 2013-09-23

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PDB ID: 2mce
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19437
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Membrane induced structure of the mammalian tachykinin neuropeptide gamma

Deposition date:

2013-08-19

Original release date:

2013-09-23

Authors:

Chandrashekar, Indu; Ganjiwale, Anjali; Cowsik, Sudha

Citation:

Citation: Chandrashekar, Indu; Ganjiwale, Anjali; Cowsik, Sudha. "Membrane-induced structure of the mammalian tachykinin neuropeptide gamma" J. Struct. Biol. 148, 315-325 (2004).
PubMed: 15522780

Assembly members:

Assembly members:
NPgamma, polymer, 21 residues, 4637.265 Da.
5-ACETYLAMINO-4-AMINO-6-(PHENETHYL-PROPYL-CARBAMOYL)-5,6-DIHYDRO-4H-PYRAN-2-CARBOXYLIC ACID, non-polymer, 389.445 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NPgamma: DAGHGQISHKRHKTDSFVGL M

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	105

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	mammalian tachykinin neuropeptide gamma	1
2	DPC	2

Entities:

Entity 1, mammalian tachykinin neuropeptide gamma 21 residues - 4637.265 Da.

1

ASP

ALA

GLY

HIS

GLY

GLN

ILE

SER

HIS

LYS

2

ARG

HIS

LYS

THR

ASP

SER

PHE

VAL

GLY

LEU

3

MET

Entity 2, DPC - C20 H27 N3 O5 - 389.445 Da.

1

DPC

Samples:

sample_1: entity 4 mg; DPC, [U-100% 2H], 250 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 2.4; pressure: 1 atm; temperature: 305 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - data analysis, processing

DIANA, Guntert, Braun and Wuthrich - refinement, structure solution

NMR spectrometers:

Bruker DRX 500 MHz