BMRB Entry 19436

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PDB ID: 2mcd
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19436
MolProbity Validation Chart

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for murine norovirus NS1/2 D94E mutant
Deposition date:
2013-08-18
Original release date:
2013-12-16
Authors:
Borin, Brendan; Krezel, Andrzej
Citation:

Citation: Borin, Brendan; Tang, Wei; Nice, Timothy; McCune, Broc; Virgin, Herbert; Krezel, Andrzej. "Murine norovirus protein NS1/2 aspartate to glutamate mutation sufficient for persistence reorients sidechain of surface exposed tryptophan within a novel structured domain"  Proteins ., .-. (2013).
PubMed: 24273131

Assembly members:

Assembly members:
entity, polymer, 98 residues, 11122.521 Da.

Natural source:

Natural source:   Common Name: norovirus   Taxonomy ID: 142786   Superkingdom: virus   Kingdom: not available   Genus/species: norovirus not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-BNK

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MRGSHHHHHHGSVSFGAPSP LSSESEDEINYMTPPEQEAQ PGALAALHAEGPLAGLPVTR SDARVLIFNEWEERKKSEPW LRLDMSDKAIFRRYPHLR

Data sets:
Data typeCount
1H chemical shifts529
15N chemical shifts75
13C chemical shifts307

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1murine norovirus NS1/2 D94E mutant1

Entities:

Entity 1, murine norovirus NS1/2 D94E mutant 98 residues - 11122.521 Da.

MRGSHHHHHHGS is a purification tag. The remainder of the sequence corresponds to residues 28-114.

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERVALSERPHEGLYALAPROSERPRO
3   LEUSERSERGLUSERGLUASPGLUILEASN
4   TYRMETTHRPROPROGLUGLNGLUALAGLN
5   PROGLYALALEUALAALALEUHISALAGLU
6   GLYPROLEUALAGLYLEUPROVALTHRARG
7   SERASPALAARGVALLEUILEPHEASNGLU
8   TRPGLUGLUARGLYSLYSSERGLUPROTRP
9   LEUARGLEUASPMETSERASPLYSALAILE
10   PHEARGARGTYRPROHISLEUARG

Related Database Links:

PDB 2MCD
GB ABS29272 ACS70958 AET79289

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks