BMRB Entry 19418

Title:
Backbone chemical shifts of the PICK1 PDZ domain with the GluA2 C-terminal
Deposition date:
2013-08-09
Original release date:
2019-12-06
Authors:
Erlendsson, Simon; Teilum, Kaare
Citation:

Citation: Erlendsson, Simon; Rathje, Mette; Heidarsson, Petur; Poulsen, Flemming; Madsen, Kenneth; Teilum, Kaare; Gether, Ulrik. "Protein interacting with C-kinase 1 (PICK1) binding promiscuity relies on unconventional PSD-95/discs-large/ZO-1 homology (PDZ) binding modes for nonclass II PDZ ligand"  J. Biol. Chem. 289, 25327-25340 (2014).
PubMed: 25023278

Assembly members:

Assembly members:
PICK1_PDZ_gluA2, polymer, 116 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Norway Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX 4T2

Data sets:
Data typeCount
13C chemical shifts220
15N chemical shifts100
1H chemical shifts100

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PICK1 PDZ gluA2 C terminal1

Entities:

Entity 1, PICK1 PDZ gluA2 C terminal 116 residues - Formula weight is not available

1   GLYSERPROGLYILEPROTHRVALPROGLY
2   LYSVALTHRLEUGLNLYSASPALAGLNASN
3   LEUILEGLYILESERILEGLYGLYGLYALA
4   GLNTYRCYSPROCYSLEUTYRILEVALGLN
5   VALPHEASPASNTHRPROALAALALEUASP
6   GLYTHRVALALAALAGLYASPGLUILETHR
7   GLYVALASNGLYARGSERILELYSGLYLYS
8   THRLYSVALGLUVALALALYSMETILEGLN
9   GLUVALLYSGLYGLUVALTHRILEHISTYR
10   ASNLYSLEUGLNALAASPPROLYSGLNLEU
11   GLUVALLEUPHEGLNGLYPROVALTYRGLY
12   ILEGLUSERVALLYSILE

Samples:

sample_1: PICK1 PDZ gluA2, [U-99% 13C; U-99% 15N], 750 uM; DSS 0.25 mM; sodium chloride 125 mM; TRIS 50 mM; Triton X 100 0,01%; H2O 90%; D2O 10%

gluA2_NMR: pH: 7.4; pressure: ambient atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicgluA2_NMR
3D CBCA(CO)NHsample_1isotropicgluA2_NMR
3D HNCOsample_1isotropicgluA2_NMR
3D HNCAsample_1isotropicgluA2_NMR
3D HNCACBsample_1isotropicgluA2_NMR
3D HN(CO)CAsample_1isotropicgluA2_NMR
2D 1H-13C HSQCsample_1isotropicgluA2_NMR

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN_analysis, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

  • Varian Unity 800 MHz
  • Varian Unity 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks