BMRB Entry 19416

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19416
MolProbity Validation Chart

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Title:
NMR Structure of Rrp7 C-terminal Domain
Deposition date:
2013-08-08
Original release date:
2013-09-23
Authors:
Lin, Jinzhong; Feng, Yingang; Ye, Keqiong
Citation:

Citation: Lin, Jinzhong; Lu, Jing; Feng, Yingang; Sun, Mengyi; Ye, Keqiong. "An RNA-binding complex involved in ribosome biogenesis contains a protein with homology to tRNA CCA-adding enzyme."  PLoS Biol. 11, .-. (2013).
PubMed: 24130456

Assembly members:

Assembly members:
entity, polymer, 46 residues, 5732.697 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PETDuet

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GPEADFYRFQVRERKKQEIN QLLSKFKEDQERIKVMKAKR KFNPYT

Data sets:
Data typeCount
13C chemical shifts218
15N chemical shifts49
1H chemical shifts350

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rrp7 C-terminal Domain1

Entities:

Entity 1, Rrp7 C-terminal Domain 46 residues - 5732.697 Da.

1   GLYPROGLUALAASPPHETYRARGPHEGLN
2   VALARGGLUARGLYSLYSGLNGLUILEASN
3   GLNLEULEUSERLYSPHELYSGLUASPGLN
4   GLUARGILELYSVALMETLYSALALYSARG
5   LYSPHEASNPROTYRTHR

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 1 mM; potassium phosphate 50 mM; sodium azide 0.05%

sample_conditions_1: ionic strength: 0.05 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

FELIX, Accelrys Software Inc. - processing

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

GB NP_009899 AHN96030 AHV79235 AHY79656 AJP37407 AJQ31893
PDB
DBJ GAA21887
EMBL CAA42385 CAY78174
REF NP_009899
SP P25368
TPG DAA07453
AlphaFold P25368

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks