BMRB Entry 19397

Name: Backbone 1H and 15N Chemical Shift Assignments for the first domain of FAT10
Published: 2014-08-25

Click here to enlarge.

PDB ID: 2mbe
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19397
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone 1H and 15N Chemical Shift Assignments for the first domain of FAT10

Deposition date:

2013-07-30

Original release date:

2014-08-25

Authors:

Wang, Wei; Lim, Liangzhong; Qin, Haina

Citation:

Citation: Wang, Wei; Lim, Liangzhong; Qin, Haina; Song, Jianxing. "Structure of FAT10 first domain" .

Assembly members:

Assembly members:
FAT10, polymer, 75 residues, 8676.306 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FAT10: LCVHVRSEEWDLMTFDANPY DSVLKIKEHVRSKTKVPVQD QVLLLGSKILKPRRSLSSYG IDKEKTIHLTLKVVK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	52
1H chemical shifts	296

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FAT10	1

Entities:

Entity 1, FAT10 75 residues - 8676.306 Da.

1

LEU

CYS

VAL

HIS

VAL

ARG

SER

GLU

TRP

2

ASP

LEU

MET

THR

PHE

ASP

ALA

ASN

PRO

TYR

3

ASP

SER

VAL

LEU

LYS

ILE

LYS

GLU

HIS

VAL

4

ARG

SER

LYS

THR

LYS

VAL

PRO

VAL

GLN

ASP

5

GLN

VAL

LEU

GLY

SER

LYS

ILE

LEU

6

LYS

PRO

ARG

SER

LEU

SER

TYR

GLY

7

ILE

ASP

LYS

GLU

LYS

THR

ILE

HIS

LEU

THR

8

LEU

LYS

VAL

LYS

Samples:

sample_1: sodium phosphate 0.01 mM; DTT 0.01 mM; FAT10, [U-100% 15N], 0.4 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 M; pH: 7.4; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - geometry optimization

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

TOPSPIN, Bruker Biospin - collection

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

Bruker Avance 800 MHz

PDB	2MBE
DBJ	BAG34855
EMBL	CAA21458 CAA73200
GB	AAD52982 AAH12472 ABM85550 AIC55799 EAX03201
REF	NP_006389 XP_004043579
SP	O15205
AlphaFold	O15205