BMRB Entry 19386

Name: parallel-stranded G-quadruplex in DNA poly-G stretches
Published: 2014-12-01

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PDB ID: 2mb2
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19386
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

parallel-stranded G-quadruplex in DNA poly-G stretches

Deposition date:

2013-07-24

Original release date:

2014-12-01

Authors:

Sengar, Anjali; Heddi, Brahim; Phan, Anh Tuan

Citation:

Citation: Sengar, Anjali; Heddi, Brahim; Phan, Anh Tuan. "Formation of G-quadruplexes in poly-G sequences: Structure of a propeller-type parallel-stranded G-quadruplex formed by a G15 stretch" Biochemistry ., .-. (2014).
PubMed: 25375976

Assembly members:

Assembly members:
DNA_(5'-D(*TP*TP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*T)-3'), polymer, 18 residues, 5805.756 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA_(5'-D(*TP*TP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*T)-3'): TTGGGGGGGGGGGGGGGT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	164

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DNA (5'-D(TPTPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPT)-3')	1

Entities:

Entity 1, DNA (5'-D(*TP*TP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*T)-3') 18 residues - 5805.756 Da.

1

DT

DG

2

DG

DT

Samples:

sample_1: DNA (5'-D(*TP*TP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*T)-3')0.1 – 2 mM; H2O 90%; D2O 10%; KCl 5 mM; KPi 5 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.0; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H JRNOESY	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR NIH v2.34, Schwieters, Kuszewski, Tjandra and Clore - geometry optimization, refinement, structure solution

NMR spectrometers:

Bruker AMX 700 MHz
Bruker AMX 600 MHz