BMRB Entry 19380

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PDB ID: 2max
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19380
MolProbity Validation Chart

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Title:
NMR structure of the RNA polymerase alpha subunit C-terminal domain from Helicobacter pylori
Deposition date:
2013-07-21
Original release date:
2014-02-13
Authors:
Borin, Brendan; Krezel, Andrzej
Citation:

Citation: Borin, Brendan; Tang, Wei; Krezel, Andrzej. "Helicobacter pylori RNA polymerase -subunit C-terminal domain shows features unique to -proteobacteria and binds NikR/DNA complexes."  Protein Sci. 23, 454-463 (2014).
PubMed: 24442709

Assembly members:

Assembly members:
RNAP_alpha_subunit_CTD, polymer, 126 residues, 12754.470 Da.

Natural source:

Natural source:   Common Name: e-proteobacteria   Taxonomy ID: 210   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Helicobacter pylori

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETBNK

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNAP_alpha_subunit_CTD: MRGSHHHHHHGSLGVFGERP IANTEYSGDYAQRDDAKDLS AKIESMNLSARCFNCLDKIG IKYVGELVLMSEEELKGVKN MGKKSYDEIAEKLNDLGYPV GTELSPEQRESLKKRLEKLE DKGGND

Data sets:
Data typeCount
13C chemical shifts340
15N chemical shifts112
1H chemical shifts736

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA polymerase alpha subunit C-terminal domain1

Entities:

Entity 1, RNA polymerase alpha subunit C-terminal domain 126 residues - 12754.470 Da.

residues 1-12 represent a non-native His6 Ni-affinity tag. Contains native residues 231-344.

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERLEUGLYVALPHEGLYGLUARGPRO
3   ILEALAASNTHRGLUTYRSERGLYASPTYR
4   ALAGLNARGASPASPALALYSASPLEUSER
5   ALALYSILEGLUSERMETASNLEUSERALA
6   ARGCYSPHEASNCYSLEUASPLYSILEGLY
7   ILELYSTYRVALGLYGLULEUVALLEUMET
8   SERGLUGLUGLULEULYSGLYVALLYSASN
9   METGLYLYSLYSSERTYRASPGLUILEALA
10   GLULYSLEUASNASPLEUGLYTYRPROVAL
11   GLYTHRGLULEUSERPROGLUGLNARGGLU
12   SERLEULYSLYSARGLEUGLULYSLEUGLU
13   ASPLYSGLYGLYASNASP

Related Database Links:

PDB 2MAX
DBJ BAJ55821 BAJ56204 BAJ58801 BAJ60323 BAM97153
EMBL CAJ99012 CAX29978 CBI65743
GB AAD06815 AAD08336 ABF85305 ACD48738 ACI27991
REF NP_208085 WP_000864476 WP_000864477 WP_000864478 WP_000864479
SP P56001 Q17ZB4 Q1CRW7 Q9ZJT5
AlphaFold P56001 Q17ZB4 Q1CRW7 Q9ZJT5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks