BMRB Entry 19377

Title:
1H, 15N, 13C Backbone and 13Cbeta Chemical Shift Assignment of mouse Interleukin-10
Deposition date:
2013-07-19
Original release date:
2013-07-23
Authors:
Kuenze, Georg; Theisgen, Stephan; Huster, Daniel
Citation:

Citation: Kuenze, Georg; Theisgen, Stephan; Huster, Daniel. "Backbone 1H, 15N, 13C and side chain 13Cbeta NMR chemical shift assignment of murine interleukin-10"  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
IL-10, polymer, 170 residues, 20160 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET41b(+)

Data sets:
Data typeCount
13C chemical shifts457
15N chemical shifts152
1H chemical shifts214

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IL-10, Chain A1
2IL-10, Chain B1
3N-terminus1

Entities:

Entity 1, IL-10, Chain A 170 residues - 20160 Da.

1   SERARGGLYGLNTYRSERARGGLUASPASN
2   ASNCYSTHRHISPHEPROVALGLYGLNSER
3   HISMETLEULEUGLULEUARGTHRALAPHE
4   SERGLNVALLYSTHRPHEPHEGLNTHRLYS
5   ASPGLNLEUASPASNILELEULEUTHRASP
6   SERLEUMETGLNASPPHELYSGLYTYRLEU
7   GLYCYSGLNALALEUSERGLUMETILEGLN
8   PHETYRLEUVALGLUVALMETPROGLNALA
9   GLULYSHISGLYPROGLUILELYSGLUHIS
10   LEUASNSERLEUGLYGLULYSLEULYSTHR
11   LEUARGMETARGLEUARGARGCYSHISARG
12   PHELEUPROCYSGLUASNLYSSERLYSALA
13   VALGLUGLNVALLYSSERASPPHEASNLYS
14   LEUGLNASPGLNGLYVALTYRLYSALAMET
15   ASNGLUPHEASPILEPHEILEASNTYRILE
16   GLUALATYRMETMETILELYSMETLYSSER
17   LEUGLUHISHISHISHISHISHISHISHIS

Samples:

sample_1: IL-10, [U-100% 13C; U-100% 15N; U-80% 2H], 1 – 2 mM; potassium phosphate 25 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.2; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz

Related Database Links:

UNP P18893
PDB
DBJ BAE31139
GB AAA39274 AAA39275 AAI20613 AAI37845 EDL39722
REF NP_034678
SP P18893
AlphaFold Q0VBJ1 P18893

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks