BMRB Entry 19377

Name: 1H, 15N, 13C Backbone and 13Cbeta Chemical Shift Assignment of mouse Interleukin-10
Published: 2013-07-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19377

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 15N, 13C Backbone and 13Cbeta Chemical Shift Assignment of mouse Interleukin-10

Deposition date:

2013-07-19

Original release date:

2013-07-23

Authors:

Kuenze, Georg; Theisgen, Stephan; Huster, Daniel

Citation:

Citation: Kuenze, Georg; Theisgen, Stephan; Huster, Daniel. "Backbone 1H, 15N, 13C and side chain 13Cbeta NMR chemical shift assignment of murine interleukin-10" Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
IL-10, polymer, 170 residues, 20160 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET41b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IL-10: SRGQYSREDNNCTHFPVGQS HMLLELRTAFSQVKTFFQTK DQLDNILLTDSLMQDFKGYL GCQALSEMIQFYLVEVMPQA EKHGPEIKEHLNSLGEKLKT LRMRLRRCHRFLPCENKSKA VEQVKSDFNKLQDQGVYKAM NEFDIFINYIEAYMMIKMKS LEHHHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	457
15N chemical shifts	152
1H chemical shifts	214

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	IL-10, Chain A	1
2	IL-10, Chain B	1
3	N-terminus	1

Entities:

Entity 1, IL-10, Chain A 170 residues - 20160 Da.

1	SER	ARG	GLY	GLN	TYR	SER	ARG	GLU	ASP	ASN
2	ASN	CYS	THR	HIS	PHE	PRO	VAL	GLY	GLN	SER
3	HIS	MET	LEU	LEU	GLU	LEU	ARG	THR	ALA	PHE
4	SER	GLN	VAL	LYS	THR	PHE	PHE	GLN	THR	LYS
5	ASP	GLN	LEU	ASP	ASN	ILE	LEU	LEU	THR	ASP
6	SER	LEU	MET	GLN	ASP	PHE	LYS	GLY	TYR	LEU
7	GLY	CYS	GLN	ALA	LEU	SER	GLU	MET	ILE	GLN
8	PHE	TYR	LEU	VAL	GLU	VAL	MET	PRO	GLN	ALA
9	GLU	LYS	HIS	GLY	PRO	GLU	ILE	LYS	GLU	HIS
10	LEU	ASN	SER	LEU	GLY	GLU	LYS	LEU	LYS	THR
11	LEU	ARG	MET	ARG	LEU	ARG	ARG	CYS	HIS	ARG
12	PHE	LEU	PRO	CYS	GLU	ASN	LYS	SER	LYS	ALA
13	VAL	GLU	GLN	VAL	LYS	SER	ASP	PHE	ASN	LYS
14	LEU	GLN	ASP	GLN	GLY	VAL	TYR	LYS	ALA	MET
15	ASN	GLU	PHE	ASP	ILE	PHE	ILE	ASN	TYR	ILE
16	GLU	ALA	TYR	MET	MET	ILE	LYS	MET	LYS	SER
17	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS	HIS	HIS

Samples:

sample_1: IL-10, [U-100% 13C; U-100% 15N; U-80% 2H], 1 – 2 mM; potassium phosphate 25 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.2; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 700 MHz
Bruker Avance 800 MHz

UNP	P18893
PDB	4X51
DBJ	BAE31139
GB	AAA39274 AAA39275 AAI20613 AAI37845 EDL39722
REF	NP_034678
SP	P18893
AlphaFold	Q0VBJ1 P18893

1	SER	ARG	GLY	GLN	TYR	SER	ARG	GLU	ASP	ASN
2	ASN	CYS	THR	HIS	PHE	PRO	VAL	GLY	GLN	SER
3	HIS	MET	LEU	LEU	GLU	LEU	ARG	THR	ALA	PHE
4	SER	GLN	VAL	LYS	THR	PHE	PHE	GLN	THR	LYS
5	ASP	GLN	LEU	ASP	ASN	ILE	LEU	LEU	THR	ASP
6	SER	LEU	MET	GLN	ASP	PHE	LYS	GLY	TYR	LEU
7	GLY	CYS	GLN	ALA	LEU	SER	GLU	MET	ILE	GLN
8	PHE	TYR	LEU	VAL	GLU	VAL	MET	PRO	GLN	ALA
9	GLU	LYS	HIS	GLY	PRO	GLU	ILE	LYS	GLU	HIS
10	LEU	ASN	SER	LEU	GLY	GLU	LYS	LEU	LYS	THR
11	LEU	ARG	MET	ARG	LEU	ARG	ARG	CYS	HIS	ARG
12	PHE	LEU	PRO	CYS	GLU	ASN	LYS	SER	LYS	ALA
13	VAL	GLU	GLN	VAL	LYS	SER	ASP	PHE	ASN	LYS
14	LEU	GLN	ASP	GLN	GLY	VAL	TYR	LYS	ALA	MET
15	ASN	GLU	PHE	ASP	ILE	PHE	ILE	ASN	TYR	ILE
16	GLU	ALA	TYR	MET	MET	ILE	LYS	MET	LYS	SER
17	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS	HIS	HIS

1	SER	ARG	GLY	GLN	TYR	SER	ARG	GLU	ASP	ASN
2	ASN	CYS	THR	HIS	PHE	PRO	VAL	GLY	GLN	SER
3	HIS	MET	LEU	LEU	GLU	LEU	ARG	THR	ALA	PHE
4	SER	GLN	VAL	LYS	THR	PHE	PHE	GLN	THR	LYS
5	ASP	GLN	LEU	ASP	ASN	ILE	LEU	LEU	THR	ASP
6	SER	LEU	MET	GLN	ASP	PHE	LYS	GLY	TYR	LEU
7	GLY	CYS	GLN	ALA	LEU	SER	GLU	MET	ILE	GLN
8	PHE	TYR	LEU	VAL	GLU	VAL	MET	PRO	GLN	ALA
9	GLU	LYS	HIS	GLY	PRO	GLU	ILE	LYS	GLU	HIS
10	LEU	ASN	SER	LEU	GLY	GLU	LYS	LEU	LYS	THR
11	LEU	ARG	MET	ARG	LEU	ARG	ARG	CYS	HIS	ARG
12	PHE	LEU	PRO	CYS	GLU	ASN	LYS	SER	LYS	ALA
13	VAL	GLU	GLN	VAL	LYS	SER	ASP	PHE	ASN	LYS
14	LEU	GLN	ASP	GLN	GLY	VAL	TYR	LYS	ALA	MET
15	ASN	GLU	PHE	ASP	ILE	PHE	ILE	ASN	TYR	ILE
16	GLU	ALA	TYR	MET	MET	ILE	LYS	MET	LYS	SER
17	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS	HIS	HIS