BMRB Entry 19364

Name: 1HN, 13C, and 15N Chemical Shift Assignments for Nupr1
Published: 2014-02-13

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19364

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1HN, 13C, and 15N Chemical Shift Assignments for Nupr1

Deposition date:

2013-07-15

Original release date:

2014-02-13

Authors:

Aguado-Llera, David; Hamidi, Tewfik; Domenech, Rosa; Gironella, Meritxell; Velazquez-Campoy, Adrian; Neira, Jose L.; Iovanna, Juan L.

Citation:

Citation: Aguado-Llera, David; Hamidi, Tewfik; Domenech, Rosa; Pantoja-Uceda, David; Gironella, Meritxell; Santoro, Jorge; Velazquez-Campoy, Adrian; Neira, Jose; Iovanna, Juan. "Deciphering the binding between Nupr1 and MSL1 and their DNA-repairing activity." PLoS ONE 8, e78101-e78101 (2013).
PubMed: 24205110

Assembly members:

Assembly members:
Nupr1, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE-30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nupr1: MRGSHHHHHHGSATFPPATS APQQPPGPEDEDSSLDESDL YSLAHSYLGGGGRKGRTKRE AAANTNRPSPGGHERKLVTK LQNSERKKRGARR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	247
15N chemical shifts	86
1H chemical shifts	78

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Nupr1	1

Entities:

Entity 1, Nupr1 93 residues - Formula weight is not available

1

MET

ARG

GLY

SER

HIS

2

GLY

SER

ALA

THR

PHE

PRO

ALA

THR

SER

3

ALA

PRO

GLN

PRO

GLY

PRO

GLU

ASP

4

GLU

ASP

SER

LEU

ASP

GLU

SER

ASP

LEU

5

TYR

SER

LEU

ALA

HIS

SER

TYR

LEU

GLY

6

GLY

ARG

LYS

GLY

ARG

THR

LYS

ARG

GLU

7

ALA

ASN

THR

ASN

ARG

PRO

SER

PRO

8

GLY

HIS

GLU

ARG

LYS

LEU

VAL

THR

LYS

9

LEU

GLN

ASN

SER

GLU

ARG

LYS

ARG

GLY

10

ALA

ARG

Samples:

sample_1: Nupr1, [U-99% 13C; U-99% 15N], 1.0 mM; acetate buffer 10 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCA intra	sample_1	isotropic	sample_conditions_1
3D HNCACB intra	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
2D CACO	sample_1	isotropic	sample_conditions_1
2D CON	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC b-carbon edited	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC g-carbon edited	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC b-carbon edited intra	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN v2.1, Bruker Biospin - collection, processing

NMRView v5.0.4, Johnson, One Moon Scientific - data analysis, peak picking

NMR spectrometers:

Bruker Avance 800 MHz

DBJ	BAG35071 BAG73451
EMBL	CAG46941
GB	AAC05336 AAC19384 AAC19385 AAD49221 AAH02434
REF	NP_036517 XP_003811192 XP_003916810 XP_003952861 XP_004057456
SP	O60356
AlphaFold	O60356