BMRB Entry 19363

Assembly:

Entities:

Entity 1, STIM1_CC1-CC2_1 82 residues - 9703.089 Da.

Residues -5-0 (i.e. GSHMAS) are a cloning artifact.

Entity 2, Orai1_1 23 residues - 2654.828 Da.

Residues -1-0 (i.e. GS) are a cloning artifact.

Samples:

sample_1: STIM1_CC1-CC2, [U-100% 15N], 0.25 mM; Orai1 3.0 mM; bis-TRIS 20 mM; TFE, [U-99% 2H], 0.175 v/v; H2O 90%; D2O 10%

sample_2: STIM1_CC1-CC2, [U-99% 13C; U-99% 15N], 0.25 mM; Orai1 3.0 mM; bis-TRIS 20 mM; TFE, [U-99% 2H], 0.175 v/v; H2O 90%; D2O 10%

sample_3: STIM1_CC1-CC2, [U-99% 13C; U-99% 15N], 0.25 mM; Orai1 3.0 mM; bis-TRIS 20 mM; TFE, [U-99% 2H], 0.175 v/v; D2O 100%

sample_4: STIM1_CC1-CC2 2.5 mM; Orai1, [U-99% 15N], 0.25 mM; bis-TRIS 20 mM; TFE, [U-99% 2H], 0.175 v/v; H2O 90%; D2O 10%

sample_5: STIM1_CC1-CC2 2.5 mM; Orai1, [U-99% 13C; U-99% 15N], 0.25 mM; bis-TRIS 20 mM; TFE, [U-99% 2H], 0.175 v/v; D2O 100%

sample_conditions_1: ionic strength: 0.02 M; pH: 5.5; pressure: 1 atm; temperature: 308 K

Experiments:

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment, peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis, geometry optimization

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - geometry optimization, refinement

NMR spectrometers:

• Bruker Avance 800 MHz
• Bruker Avance 600 MHz