BMRB Entry 19339

Name: Backbone assignment of the R8 domain of talin.
Published: 2018-09-19

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19339

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of the R8 domain of talin.

Deposition date:

2013-07-05

Original release date:

2018-09-19

Authors:

Goult, Ben; Zacharchenko, Thomas; Bate, Neil; Critchley, David; Barsukov, Igor

Citation:

Citation: Zacharchenko, Thomas; Qian, Xiaolan; Goult, Benjamin; Jethwa, Devina; Almeida, Teresa; Ballestrem, Christoph; Critchley, David; Lowy, Douglas; Barsukov, Igor. "LD Motif Recognition by Talin: Structure of the Talin-DLC1 Complex." Structure 24, 1130-1141 (2016).
PubMed: 27265849

Assembly members:

Assembly members:
R8, polymer, 133 residues, 13970.6 Da.

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET151-TOPO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
R8: GIDPFTQQGLVEPTQFARAN QAIQMACQSLGEPGCTQAQV LSAATIVAKHTSALCNSCRL ASARTANPTAKRQFVQSAKE VANSTANLVKTIKALDGDFT EENRAQCRAATAPLLEAVDN LSAFASNPEFSSV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	392
15N chemical shifts	125
1H chemical shifts	125

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	R8	1

Entities:

Entity 1, R8 133 residues - 13970.6 Da.

Residues 1-6 (1452-1457) represent a non-native affinity tag

1

GLY

ILE

ASP

PRO

PHE

THR

GLN

GLY

LEU

2

VAL

GLU

PRO

THR

GLN

PHE

ALA

ARG

ALA

ASN

3

GLN

ALA

ILE

GLN

MET

ALA

CYS

GLN

SER

LEU

4

GLY

GLU

PRO

GLY

CYS

THR

GLN

ALA

GLN

VAL

5

LEU

SER

ALA

THR

ILE

VAL

ALA

LYS

HIS

6

THR

SER

ALA

LEU

CYS

ASN

SER

CYS

ARG

LEU

7

ALA

SER

ALA

ARG

THR

ALA

ASN

PRO

THR

ALA

8

LYS

ARG

GLN

PHE

VAL

GLN

SER

ALA

LYS

GLU

9

VAL

ALA

ASN

SER

THR

ALA

ASN

LEU

VAL

LYS

10

THR

ILE

LYS

ALA

LEU

ASP

GLY

ASP

PHE

THR

11

GLU

ASN

ARG

ALA

GLN

CYS

ARG

ALA

12

THR

ALA

PRO

LEU

GLU

ALA

VAL

ASP

ASN

13

LEU

SER

ALA

PHE

ALA

SER

ASN

PRO

GLU

PHE

14

SER

VAL

Samples:

sample_1: talin R8, [U-100% 13C; U-100% 15N], 0.8 ± 0.05 mM; DTT 2 ± 0.05 mM; sodium phosphate 20 ± 0.05 mM; sodium chloride 50 ± 0.05 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.2, Bruker Biospin - collection, processing

Analysis v2.2.2, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker DRX 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks