BMRB Entry 19331

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19331
MolProbity Validation Chart

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Title:
Solution NMR Structure of Zinc finger protein Eos from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR7992A
Deposition date:
2013-06-28
Original release date:
2013-09-03
Authors:
Pulavarti, Surya VSRK; Mills, Jeffrey; Wang, Dongyan; Kohan, Eitan; Hamilton, Keith; Xiao, Rong; Everett, John; Acton, Thomas; Montelione, Gaetano; Szyperski, Thomas
Citation:

Citation: Pulavarti, Surya VSRK; Mills, Jeffrey; Wang, Dongyan; Kohan, Eitan; Hamilton, Keith; Xiao, Rong; Everett, John; Acton, Thomas; Montelione, Gaetano; Szyperski, Thomas; NESG, NESG. "Solution NMR Structure of Zinc finger protein Eos from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR7992A"  To be published ., .-..

Assembly members:

Assembly members:
HR7992A, polymer, 74 residues, 8322.126 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15_NESG

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HR7992A: MGHHHHHHSHMPNGKLKCDV CGMVCIGPNVLMVHKRSHTG ERPFHCNQCGASFTQKGNLL RHIKLHSGEKPFKX

Data sets:
Data typeCount
13C chemical shifts265
15N chemical shifts76
1H chemical shifts425

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HR7992A1
2ZINC ION2

Entities:

Entity 1, HR7992A 74 residues - 8322.126 Da.

1   METGLYHISHISHISHISHISHISSERHIS
2   METPROASNGLYLYSLEULYSCYSASPVAL
3   CYSGLYMETVALCYSILEGLYPROASNVAL
4   LEUMETVALHISLYSARGSERHISTHRGLY
5   GLUARGPROPHEHISCYSASNGLNCYSGLY
6   ALASERPHETHRGLNLYSGLYASNLEULEU
7   ARGHISILELYSLEUHISSERGLYGLULYS
8   PROPHELYSX

Entity 2, ZINC ION - 65.409 Da.

1   ZN

Samples:

sample_1: HR7992A.010, [U-100% 13C; U-100% 15N], 1.0 mM; DTT 5 mM; NaCl 100 mM; Tris-HCl pH 7.5 10 mM; NaN3 0.02%; H2O 90%; D2O 10%

sample_2: HR7992A.011, [5% 13C; U-100% 15N], 1 mM; Tris-HCl pH 7.5 10 mM; NaCl 100 mM; NaN3 0.02 mM; DTT 5 mM; ZINC ION 4 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C(CT) Aliphatic (28 ms)sample_2isotropicsample_conditions_1
2D 1H-13C(CT) Aliphaticsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
gft43d_hcch_cosy aliphaticsample_1isotropicsample_conditions_1
gft43d_hcch_cosy aromaticsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQC (His longrange)sample_1isotropicsample_conditions_1
2D 1H-13C(CT)HSQC aromaticsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinemen,structure solution,geometry optimization

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - refinement,geometry optimization,structure solution

AutoStruct v2.1, Huang, Tejero, Powers and Montelione - data analysis,refinement

AutoAssign v2.1, Zimmerman, Moseley, Kulikowski and Montelione - data analysis,chemical shift assignment

XEASY, Bartels et al. - data analysis,peak picking,chemical shift assignment

VNMRJ, Varian - collection

TALOS+, Shen, Cornilescu, Delaglio and Bax - geometry optimization

PROSA, Guntert - processing

PSVS, Bhattacharya, Montelione - structure validation

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAC33191 BAG37131 BAG63014 BAI46179
EMBL CAC59948 CAH92275
GB AAC00513 AAF09441 AAG39221 AAH73084 AAI38607
REF NP_001100386 NP_001116930 NP_001126330 NP_001295240 NP_035900
SP P81183 Q9UKS7
TPG DAA32460
AlphaFold P81183 Q9UKS7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks