BMRB Entry 19325

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19325

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Title:
1H, 13C, and 15N Chemical Shift Assignments for a proteasome related subunit
Deposition date:
2013-06-27
Original release date:
2014-02-13
Authors:
Wu, Yujie; Hu, Yunfei; Jin, Changwen
Citation:

Citation: Wu, Yujie; Hu, Yunfei; Jin, Changwen. "(1)H, (13)C and (15)N resonance assignments of the VWA domain of Saccharomyces cerevisiae Rpn10, a regulatory subunit of 26S proteasome."  Biomol. NMR Assignments ., .-. (2013).
PubMed: 24037519

Assembly members:

Assembly members:
26s_proteasome_subunit, polymer, 207 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
26s_proteasome_subunit: MVLEATVLVIDNSEYSRNGD FPRTRFEAQIDSVEFIFQAK RNSNPENTVGLISGAGANPR VLSTFTAEFGKILAGLHDTQ IEGKLHMATALQIAQLTLKH RQNKVQHQRIVAFVCSPISD SRDELIRLAKTLKKNNVAVD IINFGEIEQNTELLDEFIAA VNNPQEETSHLLTVTPGPRL LYENIASSPIILEEGSSGMG AFGGSGG

Data sets:
Data typeCount
13C chemical shifts811
15N chemical shifts208
1H chemical shifts1332

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
126S proteasome subunit monomer1

Entities:

Entity 1, 26S proteasome subunit monomer 207 residues - Formula weight is not available

1   METVALLEUGLUALATHRVALLEUVALILE
2   ASPASNSERGLUTYRSERARGASNGLYASP
3   PHEPROARGTHRARGPHEGLUALAGLNILE
4   ASPSERVALGLUPHEILEPHEGLNALALYS
5   ARGASNSERASNPROGLUASNTHRVALGLY
6   LEUILESERGLYALAGLYALAASNPROARG
7   VALLEUSERTHRPHETHRALAGLUPHEGLY
8   LYSILELEUALAGLYLEUHISASPTHRGLN
9   ILEGLUGLYLYSLEUHISMETALATHRALA
10   LEUGLNILEALAGLNLEUTHRLEULYSHIS
11   ARGGLNASNLYSVALGLNHISGLNARGILE
12   VALALAPHEVALCYSSERPROILESERASP
13   SERARGASPGLULEUILEARGLEUALALYS
14   THRLEULYSLYSASNASNVALALAVALASP
15   ILEILEASNPHEGLYGLUILEGLUGLNASN
16   THRGLULEULEUASPGLUPHEILEALAALA
17   VALASNASNPROGLNGLUGLUTHRSERHIS
18   LEULEUTHRVALTHRPROGLYPROARGLEU
19   LEUTYRGLUASNILEALASERSERPROILE
20   ILELEUGLUGLUGLYSERSERGLYMETGLY
21   ALAPHEGLYGLYSERGLYGLY

Samples:

sample_1: 26s_proteasome_subunit, [U-100% 13C; U-100% 15N], 1.0 mM; MES 30 mM; DTT 10 mM; sodium chloride 50 mM; DSS 0.03%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.08 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Zhengrong and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

CARA, Keller and Wuthrich - data analysis

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAA11207 GAA23917
EMBL CAY80217
GB AAB68355 AAS56615 AHY77896 AJP39292 AJU16491
REF NP_012070
SP P38886
TPG DAA06893
AlphaFold P38886

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks