BMRB Entry 19322
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19322
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Robertson, Ian; Handford, Penny; Redfield, Christina. "NMR Spectroscopic and Bioinformatic Analyses of the LTBP1 C-Terminus Reveal a Highly Dynamic Domain Organisation." PLoS ONE 9, e87125-e87125 (2014).
PubMed: 24489852
Assembly members:
cb14TB3, polymer, 117 residues, Formula weight is not available
TB3E3, polymer, 172 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE30
Entity Sequences (FASTA):
cb14TB3: SADMDECQDPSSCIDGQCVN
TEGSYNCFCTHPMVLDASEK
RCIRPAESNEQIEETDVYQD
LCWEHLSDEYVCSRPLVGKQ
TTYTECCCLYGEAWGMQCAL
CPLKDSDDYAQLCNIPV
TB3E3: MRGSHHHHHHGSIEGRSARP
AESNEQIEETDVYQDLCWEH
LSDEYVCSRPLVGKQTTYTE
CCCLYGEAWGMQCALCPLKD
SDDYAQLCNIPVTGRRQPYG
RDALVDFSEQYTPEADPYFI
QDRFLNSFEELQAEECGILN
GCENGRCVRVQEGYTCDCFD
GYHLDTAKMTCV
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 236 |
| 1H chemical shifts | 489 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cb14TB3 | 1 |
| 2 | TB3E3 | 2 |
| 3 | CALCIUM ION | 3 |
Entities:
Entity 1, cb14TB3 117 residues - Formula weight is not available
| 1 | SER | ALA | ASP | MET | ASP | GLU | CYS | GLN | ASP | PRO | ||||
| 2 | SER | SER | CYS | ILE | ASP | GLY | GLN | CYS | VAL | ASN | ||||
| 3 | THR | GLU | GLY | SER | TYR | ASN | CYS | PHE | CYS | THR | ||||
| 4 | HIS | PRO | MET | VAL | LEU | ASP | ALA | SER | GLU | LYS | ||||
| 5 | ARG | CYS | ILE | ARG | PRO | ALA | GLU | SER | ASN | GLU | ||||
| 6 | GLN | ILE | GLU | GLU | THR | ASP | VAL | TYR | GLN | ASP | ||||
| 7 | LEU | CYS | TRP | GLU | HIS | LEU | SER | ASP | GLU | TYR | ||||
| 8 | VAL | CYS | SER | ARG | PRO | LEU | VAL | GLY | LYS | GLN | ||||
| 9 | THR | THR | TYR | THR | GLU | CYS | CYS | CYS | LEU | TYR | ||||
| 10 | GLY | GLU | ALA | TRP | GLY | MET | GLN | CYS | ALA | LEU | ||||
| 11 | CYS | PRO | LEU | LYS | ASP | SER | ASP | ASP | TYR | ALA | ||||
| 12 | GLN | LEU | CYS | ASN | ILE | PRO | VAL |
Entity 2, TB3E3 172 residues - Formula weight is not available
| 1 | MET | ARG | GLY | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | GLY | SER | ILE | GLU | GLY | ARG | SER | ALA | ARG | PRO | ||||
| 3 | ALA | GLU | SER | ASN | GLU | GLN | ILE | GLU | GLU | THR | ||||
| 4 | ASP | VAL | TYR | GLN | ASP | LEU | CYS | TRP | GLU | HIS | ||||
| 5 | LEU | SER | ASP | GLU | TYR | VAL | CYS | SER | ARG | PRO | ||||
| 6 | LEU | VAL | GLY | LYS | GLN | THR | THR | TYR | THR | GLU | ||||
| 7 | CYS | CYS | CYS | LEU | TYR | GLY | GLU | ALA | TRP | GLY | ||||
| 8 | MET | GLN | CYS | ALA | LEU | CYS | PRO | LEU | LYS | ASP | ||||
| 9 | SER | ASP | ASP | TYR | ALA | GLN | LEU | CYS | ASN | ILE | ||||
| 10 | PRO | VAL | THR | GLY | ARG | ARG | GLN | PRO | TYR | GLY | ||||
| 11 | ARG | ASP | ALA | LEU | VAL | ASP | PHE | SER | GLU | GLN | ||||
| 12 | TYR | THR | PRO | GLU | ALA | ASP | PRO | TYR | PHE | ILE | ||||
| 13 | GLN | ASP | ARG | PHE | LEU | ASN | SER | PHE | GLU | GLU | ||||
| 14 | LEU | GLN | ALA | GLU | GLU | CYS | GLY | ILE | LEU | ASN | ||||
| 15 | GLY | CYS | GLU | ASN | GLY | ARG | CYS | VAL | ARG | VAL | ||||
| 16 | GLN | GLU | GLY | TYR | THR | CYS | ASP | CYS | PHE | ASP | ||||
| 17 | GLY | TYR | HIS | LEU | ASP | THR | ALA | LYS | MET | THR | ||||
| 18 | CYS | VAL |
Entity 3, CALCIUM ION - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_LTBP1-cb14TB3-1: cb14TB3, [U-100% 15N], 2 – 4 mM; TB3E3, [U-100% 15N], 2 – 4 mM; Calcium Chloride 20 mM
sample_LTBP1-cb14TB3-2: cb14TB3, [U-100% 15N], 2 – 3 mM; TB3E3, [U-100% 15N], 2 – 3 mM
sample_conditions-1: ionic strength: 60 mM; pH: 5.9; pressure: 1 atm; temperature: 273 K
sample_conditions-2: ionic strength: 0 mM; pH: 6.9; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_LTBP1-cb14TB3-1 | isotropic | sample_conditions-1 |
| 3D 1H-15N NOESY | sample_LTBP1-cb14TB3-1 | isotropic | sample_conditions-1 |
| 3D 1H-15N TOCSY | sample_LTBP1-cb14TB3-1 | isotropic | sample_conditions-1 |
| 2D 1H-15N HSQC | sample_LTBP1-cb14TB3-2 | isotropic | sample_conditions-2 |
| 3D 1H-15N NOESY | sample_LTBP1-cb14TB3-2 | isotropic | sample_conditions-2 |
| 3D 1H-15N TOCSY | sample_LTBP1-cb14TB3-2 | isotropic | sample_conditions-2 |
| 3D 1H-15N HSQC-NOESY-HSQC | sample_LTBP1-cb14TB3-1 | isotropic | sample_conditions-1 |
| 3D 1H-15N HSQC-NOESY-HSQC | sample_LTBP1-cb14TB3-2 | isotropic | sample_conditions-2 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CCPN_Analysis, CCPN - chemical shift assignment
NMR spectrometers:
- Home built OMEGA 500 MHz
- Home built OMEGA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks