BMRB Entry 19318

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19318

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Title:
Chemical shifts of the CPAP-interacting epitope of Danio rerio STIL
Deposition date:
2013-06-24
Original release date:
2013-09-13
Authors:
Hatzopoulos, Georgios; Vakonakis, Ioannis
Citation:

Citation: Hatzopoulos, Georgios; Erat, Michele; Cutts, Erin; Rogala, Kacper; Slater, Leanne; Stansfeld, Philip; Vakonakis, Ioannis. "Structural Analysis of the G-Box Domain of the Microcephaly Protein CPAP Suggests a Role in Centriole Architecture."  Structure 21, 2069-2077 (2013).
PubMed: 24076405

Assembly members:

Assembly members:
drSTIL_N6, polymer, 60 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Zebra fish   Taxonomy ID: 7955   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Danio rerio

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16-3c

Entity Sequences (FASTA):

Entity Sequences (FASTA):
drSTIL_N6: GPLGSCYQNKLSISDHDSGV EDEDLSPRPSPNPHPVSQQT KRVHPLVPELSMVLDGSFLD

Data sets:
Data typeCount
13C chemical shifts172
15N chemical shifts50
1H chemical shifts112

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1drSTIL1

Entities:

Entity 1, drSTIL 60 residues - Formula weight is not available

Residues 1-7 represent a cloning artifact

1   GLYPROLEUGLYSERCYSTYRGLNASNLYS
2   LEUSERILESERASPHISASPSERGLYVAL
3   GLUASPGLUASPLEUSERPROARGPROSER
4   PROASNPROHISPROVALSERGLNGLNTHR
5   LYSARGVALHISPROLEUVALPROGLULEU
6   SERMETVALLEUASPGLYSERPHELEUASP

Samples:

sample_1: drSTIL_N6, [U-98% 13C; U-98% 15N], 0.25 mM; DSS 0.05 mM; sodium chloride 0.15 mM; sodium phosphate 0.02 mM; sodium azide 0.01%; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.21 M; pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HCA(CO)Nsample_1isotropicsample_conditions_1
3D HBHANHsample_1isotropicsample_conditions_1

Software:

Omega Spectrometer Operating Software vBeta 6.03b2, General Electric - collection

NMRPipe v2.4 Rev 2006.095.11.35, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PIPP v4.3.7, Garrett - data analysis

NMR spectrometers:

  • GE OMEGA PSG 600 MHz

Related Database Links:

UNP B5DDH8
PDB
AlphaFold B5DDH8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks