BMRB Entry 19317

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19317
MolProbity Validation Chart

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Title:
Solution structure of HRDC1 domain of RecQ helicase from Deinococcus radiodurans
Deposition date:
2013-06-24
Original release date:
2013-07-08
Authors:
Liu, Shanshan; Zhang, Wen; Gao, Zengqiang; Ming, Qianqian; Hou, Haifeng; Lan, Wenxian; Wu, Houming; Cao, Chunyang; Dong, Yuhui
Citation:

Citation: Liu, Shanshan; Zhang, Wen; Gao, Zengqiang; Ming, Qianqian; Hou, Haifeng; Lan, Wenxian; Wu, Houming; Cao, Chunyang; Dong, Yuhui. "NMR structure of the N-terminal-most HRDC1 domain of RecQ helicase from Deinococcus radiodurans."  FEBS Lett. 587, 2635-2642 (2013).
PubMed: 23831579

Assembly members:

Assembly members:
DNA_helicase_RecQ, polymer, 75 residues, 8054.224 Da.

Natural source:

Natural source:   Common Name: Deinococcus radiodurans   Taxonomy ID: 1299   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Deinococcus radiodurans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA_helicase_RecQ: HDAPLFEALRAWRLQKAKEL SLPPYTIFHDATLKTIAELR PGSHATLGTVSGVGGRKLAA YGDEVLQVVRDSSGG

Data sets:
Data typeCount
13C chemical shifts283
15N chemical shifts70
1H chemical shifts511

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA helicase RecQ1

Entities:

Entity 1, DNA helicase RecQ 75 residues - 8054.224 Da.

1   HISASPALAPROLEUPHEGLUALALEUARG
2   ALATRPARGLEUGLNLYSALALYSGLULEU
3   SERLEUPROPROTYRTHRILEPHEHISASP
4   ALATHRLEULYSTHRILEALAGLULEUARG
5   PROGLYSERHISALATHRLEUGLYTHRVAL
6   SERGLYVALGLYGLYARGLYSLEUALAALA
7   TYRGLYASPGLUVALLEUGLNVALVALARG
8   ASPSERSERGLYGLY

Samples:

sample: HRDC1, [U-98% 13C; U-98% 15N], 2 mM; phosphate buffer 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsampleisotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsampleisotropicsample_conditions_1
3D 1H-13C NOESY aromaticsampleisotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 18124
PDB

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