BMRB Entry 19294

Name: DNA-binding domain of T. brucei telomeric protein tbTRF
Published: 2014-07-14

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PDB ID: 2m9h
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19294
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

DNA-binding domain of T. brucei telomeric protein tbTRF

Deposition date:

2013-06-09

Original release date:

2014-07-14

Authors:

LI, Xiaohua; YE, Fei; ZHANG, Mingjie; ZHAO, Yanxiang

Citation:

Citation: LI, Xiaohua; YE, Fei; ZHANG, Mingjie; ZHAO, Yanxiang. "DNA-binding domain of T. brucei telomeric protein tbTRF" .

Assembly members:

Assembly members:
entity, polymer, 106 residues, 12054.533 Da.

Natural source:

Natural source: Common Name: dog Taxonomy ID: 9615 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Canis familiaris

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GPGSLSNFANVGVGTSSGKQ KRYKFSASEDEAIIKGLARF TKGQQRFQQIYYAYRSVWHP ARTVSQLYDHWRGTLRYKVI QQQGYRGKNSVAARSPEKAS NMENNE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	98
1H chemical shifts	97

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DNA-binding domain of T. brucei telomeric protein tbTRF	1

Entities:

Entity 1, DNA-binding domain of T. brucei telomeric protein tbTRF 106 residues - 12054.533 Da.

1

GLY

PRO

GLY

SER

LEU

SER

ASN

PHE

ALA

ASN

2

VAL

GLY

VAL

GLY

THR

SER

GLY

LYS

GLN

3

LYS

ARG

TYR

LYS

PHE

SER

ALA

SER

GLU

ASP

4

GLU

ALA

ILE

LYS

GLY

LEU

ALA

ARG

PHE

5

THR

LYS

GLY

GLN

ARG

PHE

GLN

ILE

6

TYR

ALA

TYR

ARG

SER

VAL

TRP

HIS

PRO

7

ALA

ARG

THR

VAL

SER

GLN

LEU

TYR

ASP

HIS

8

TRP

ARG

GLY

THR

LEU

ARG

TYR

LYS

VAL

ILE

9

GLN

GLY

TYR

ARG

GLY

LYS

ASN

SER

10

VAL

ALA

ARG

SER

PRO

GLU

LYS

ALA

SER

11

ASN

MET

GLU

ASN

GLU

Samples:

sample_1: DNA-binding domain of T. brucei telomeric protein tbTRF mM; potassium phosphate 100 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 5.6; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

Varian INOVA 500 MHz

PDB	2M9H
EMBL	CBH16451
GB	AAX86992 EAN78659
REF	XP_011778715 XP_823487