BMRB Entry 19285

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19285
MolProbity Validation Chart

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Title:
Optimized Ratiometric Calcium Sensors For Functional In Vivo Imaging of Neurons and T-Lymphocytes
Deposition date:
2013-06-04
Original release date:
2014-01-02
Authors:
Russo, Luigi; Becker, Stefan; Griesinger, Christian
Citation:

Citation: Thestrup, Thomas; Litzlbauer, Julia; Bartholomaus, Ingo; Mues, Marsilius; Russo, Luigi; Dana, Hod; Kovalchuk, Yuri; Liang, Yajie; Kalamakis, Georgios; Laukat, Yvonne; Becker, Stefan; Witte, Gregor; Geiger, Anselm; Allen, Taylor; Rome, Lawrence; Chen, Tsai-Wen; Kim, Douglas; Garaschuk, Olga; Griesinger, Christian; Griesbeck, Oliver. "Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes."  Nat. Methods 11, 175-182 (2014).
PubMed: 24390440

Assembly members:

Assembly members:
entity, polymer, 69 residues, 8066.708 Da.

Natural source:

Natural source:   Common Name: oyster toadfish   Taxonomy ID: 8068   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Opsanus tau

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSETb

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: SEEELSECFRIFDKDGNGFI DREEFGDIIRLTGEQLTDED VDEIFGDSDTDKNGRIDFDE FLKMMENVQ

Data sets:
Data typeCount
13C chemical shifts199
15N chemical shifts69
1H chemical shifts143

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Calcium Sensor1

Entities:

Entity 1, Calcium Sensor 69 residues - 8066.708 Da.

1   SERGLUGLUGLULEUSERGLUCYSPHEARG
2   ILEPHEASPLYSASPGLYASNGLYPHEILE
3   ASPARGGLUGLUPHEGLYASPILEILEARG
4   LEUTHRGLYGLUGLNLEUTHRASPGLUASP
5   VALASPGLUILEPHEGLYASPSERASPTHR
6   ASPLYSASNGLYARGILEASPPHEASPGLU
7   PHELEULYSMETMETGLUASNVALGLN

Samples:

sample_1: Calcium sensor, [U-13C; U-15N], ; H2O 90%; D2O 10%; TRIS 1 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1

Software:

CS-Rosetta, Shen, Vernon, Baker and Bax - structure solution

NMR spectrometers:

  • Bruker DRX 700 MHz
  • Bruker DRX 900 MHz

Related Database Links:

PDB
GB AHD25944

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SPARKY: Backbone or all simulated peaks