BMRB Entry 19272

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19272

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Title:
1H, 15N, 13C resonance assignments of Yersinia phosphatase YopH
Deposition date:
2013-05-28
Original release date:
2013-05-29
Authors:
Whittier, Sean; Loria, J.
Citation:

Citation: Whittier, Sean; Loria, J.. "1H, 15N, 13C resonance assignments of Yersinia phosphatase YopH"  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
YopH, polymer, 306 residues, 33513 Da.

Natural source:

Natural source:   Common Name: Enterobacteria   Taxonomy ID: 632   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Yersinia pestis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET19b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
YopH: MRERPHTSGHHGAGEARATA PSTVSPYGPEARAELSSRLT TLRNTLAPATNDPRYLQACG GEKLNRFRDIQCRRQTAVRA DLNANYIQVGNTRTIACQYP LQSQLESHFRMLAENRTPVL AVLASSSEIANQRFGMPDYF RQSGTYGSITVESKMTQQVG LGDGIMADMYTLTIREAGQK TISVPVVHVGNWPDQTAVSS EVTKALASLVDQTAETKRNM YESKGSSAVADDSKLRPVIH CRAGVGRTAQLIGAMCMNDS RNSQLSVEDMVSQMRVQRNG IMVQKDEQLDVLIKLAEGQG RPLLNS

Data sets:
Data typeCount
13C chemical shifts760
15N chemical shifts271
1H chemical shifts271

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YopH1

Entities:

Entity 1, YopH 306 residues - 33513 Da.

1   METARGGLUARGPROHISTHRSERGLYHIS
2   HISGLYALAGLYGLUALAARGALATHRALA
3   PROSERTHRVALSERPROTYRGLYPROGLU
4   ALAARGALAGLULEUSERSERARGLEUTHR
5   THRLEUARGASNTHRLEUALAPROALATHR
6   ASNASPPROARGTYRLEUGLNALACYSGLY
7   GLYGLULYSLEUASNARGPHEARGASPILE
8   GLNCYSARGARGGLNTHRALAVALARGALA
9   ASPLEUASNALAASNTYRILEGLNVALGLY
10   ASNTHRARGTHRILEALACYSGLNTYRPRO
11   LEUGLNSERGLNLEUGLUSERHISPHEARG
12   METLEUALAGLUASNARGTHRPROVALLEU
13   ALAVALLEUALASERSERSERGLUILEALA
14   ASNGLNARGPHEGLYMETPROASPTYRPHE
15   ARGGLNSERGLYTHRTYRGLYSERILETHR
16   VALGLUSERLYSMETTHRGLNGLNVALGLY
17   LEUGLYASPGLYILEMETALAASPMETTYR
18   THRLEUTHRILEARGGLUALAGLYGLNLYS
19   THRILESERVALPROVALVALHISVALGLY
20   ASNTRPPROASPGLNTHRALAVALSERSER
21   GLUVALTHRLYSALALEUALASERLEUVAL
22   ASPGLNTHRALAGLUTHRLYSARGASNMET
23   TYRGLUSERLYSGLYSERSERALAVALALA
24   ASPASPSERLYSLEUARGPROVALILEHIS
25   CYSARGALAGLYVALGLYARGTHRALAGLN
26   LEUILEGLYALAMETCYSMETASNASPSER
27   ARGASNSERGLNLEUSERVALGLUASPMET
28   VALSERGLNMETARGVALGLNARGASNGLY
29   ILEMETVALGLNLYSASPGLUGLNLEUASP
30   VALLEUILELYSLEUALAGLUGLYGLNGLY
31   ARGPROLEULEUASNSER

Samples:

sample_1: YopH, [U-100% 13C; U-100% 15N; U-80% 2H], 450 uM; Bis-Tris propane 20 mM; sodium chloride 100 mM; EDTA 1 mM; DTT 3 mM; sodium azide 0.02 w/v; D2O, [U-2H], 7%; H2O 93%

sample_conditions_1: ionic strength: 0.11 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ, Varian - collection

CARA, Keller & Wuthrich - chemical shift assignment

NMR spectrometers:

  • Varian Uniform NMR System 900 MHz
  • Varian Uniform NMR System 900 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 600 MHz
  • Varian Uniform NMR System 800 MHz

Related Database Links:

PDB
EMBL CAA68629 CAB54944 CAF25437 CAL10072 CBW54720
GB AAA19860 AAC62606 AAC69768 AAD16847 AAK69246
REF NP_052424 NP_395201 NP_783699 NP_857779 NP_857910
SP P08538 P15273
AlphaFold P08538 P15273

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks