BMRB Entry 19270

Name: Backbone assignment of the Sigma-1 receptor chaperone domain
Published: 2014-02-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19270

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of the Sigma-1 receptor chaperone domain

Deposition date:

2013-05-27

Original release date:

2014-02-13

Authors:

Ortega-Roldan, Jose L.; Schnell, Jason

Citation:

Citation: Ortega-Roldan, Jose Luis; Ossa, Felipe; Schnell, Jason. "Characterization of the human sigma-1 receptor chaperone domain structure and binding immunoglobulin protein (BiP) interactions." J. Biol. Chem. 288, 21448-21457 (2013).
PubMed: 23760505

Assembly members:

Assembly members:
S1ch, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pCold-I

Entity Sequences (FASTA):

Entity Sequences (FASTA):
S1ch: MNHKVHHHHHHIEGRHMGSR GHSGRYWAEISDTIISGTFH QWREGTTKSEVFYPGETVVH GPGEATAVEWGPNTWMVEYG RGVIPSTLAFALADTVFSTQ DFLTLFYTLRSYARGLRLEL TTYLFGQDP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	297
15N chemical shifts	105
1H chemical shifts	214

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	S1ch	1

Entities:

Entity 1, S1ch 129 residues - Formula weight is not available

1

MET

ASN

HIS

LYS

VAL

HIS

2

HIS

ILE

GLU

GLY

ARG

HIS

MET

GLY

SER

ARG

3

GLY

HIS

SER

GLY

ARG

TYR

TRP

ALA

GLU

ILE

4

SER

ASP

THR

ILE

SER

GLY

THR

PHE

HIS

5

GLN

TRP

ARG

GLU

GLY

THR

LYS

SER

GLU

6

VAL

PHE

TYR

PRO

GLY

GLU

THR

VAL

HIS

7

GLY

PRO

GLY

GLU

ALA

THR

ALA

VAL

GLU

TRP

8

GLY

PRO

ASN

THR

TRP

MET

VAL

GLU

TYR

GLY

9

ARG

GLY

VAL

ILE

PRO

SER

THR

LEU

ALA

PHE

10

ALA

LEU

ALA

ASP

THR

VAL

PHE

SER

THR

GLN

11

ASP

PHE

LEU

THR

LEU

PHE

TYR

THR

LEU

ARG

12

SER

TYR

ALA

ARG

GLY

LEU

ARG

LEU

GLU

LEU

13

THR

TYR

LEU

PHE

GLY

GLN

ASP

PRO

Samples:

sample_1: S1ch, [U-100% 13C; U-100% 15N; U-80% 2H], 0.27 mM; S1ch 95%; S1ch 5%

sample_2: S1ch, [U-100% 13C; U-100% 15N], 0.27 mM; S1ch 95%; S1ch 5%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DRX 500 MHz

EMBL	CAG33356
GB	AAB50402 AAB51238 AAC04507 AAH04899 ABM83807
REF	NP_001244806 NP_001269135 NP_001269136 NP_005857 XP_002743083
SP	Q99720
AlphaFold	Q99720