BMRB Entry 19267

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19267

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Title:
Backbone Assignment of Outer Envelope Protein 16kDa
Deposition date:
2013-05-26
Original release date:
2014-02-13
Authors:
Zook, James; Molugu, Trivikram; Jacobsen, Neil; Lin, Guangxin; Brown, Michael; Fromme, Petra
Citation:

Citation: Zook, James; Molugu, Trivikram; Jacobsen, Neil; Lin, Guangxin; Soll, Jurgen; Cherry, Brian; Brown, Michael; Fromme, Petra. "High-resolution NMR reveals secondary structure and folding of amino acid transporter from outer chloroplast membrane."  PLoS ONE 8, e78116-e78116 (2013).
PubMed: 24205117

Assembly members:

Assembly members:
OEP16, polymer, 154 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Eudicots   Taxonomy ID: 3888   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Pisum sativum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETbPrp

Entity Sequences (FASTA):

Entity Sequences (FASTA):
OEP16: MPRSSFSGSLSSPKLDVVID MGNPFLNLTVDGFLKIGAVA ATRSVAEDTFHIIRKGSISS NDFEKSLKKMCKEGAYWGAI AGVYVGMEYGVERIRGTRDW KNAMFGGAVTGALVSAASNN KKDKIAVDAITGAAIATAAE FINYLTLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts443
15N chemical shifts150
1H chemical shifts271

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1OEP161

Entities:

Entity 1, OEP16 154 residues - Formula weight is not available

This is a membrane protein with a non-native, C-terminal 6x Histidine tag

1   METPROARGSERSERPHESERGLYSERLEU
2   SERSERPROLYSLEUASPVALVALILEASP
3   METGLYASNPROPHELEUASNLEUTHRVAL
4   ASPGLYPHELEULYSILEGLYALAVALALA
5   ALATHRARGSERVALALAGLUASPTHRPHE
6   HISILEILEARGLYSGLYSERILESERSER
7   ASNASPPHEGLULYSSERLEULYSLYSMET
8   CYSLYSGLUGLYALATYRTRPGLYALAILE
9   ALAGLYVALTYRVALGLYMETGLUTYRGLY
10   VALGLUARGILEARGGLYTHRARGASPTRP
11   LYSASNALAMETPHEGLYGLYALAVALTHR
12   GLYALALEUVALSERALAALASERASNASN
13   LYSLYSASPLYSILEALAVALASPALAILE
14   THRGLYALAALAILEALATHRALAALAGLU
15   PHEILEASNTYRLEUTHRLEUGLUHISHIS
16   HISHISHISHIS

Samples:

sample_1: sodium chloride 100 mM; sodium phosphate 20 mM; EDTA 1 mM; beta-mercaptoethanol 1 mM; glycerol 10 v/v; SDS 0.4 w/v; sodium azide 0.02 w/v; OEP16, [U-99% 15N], 1.5 mM; H2O 90%; D2O 10%

sample_2: sodium chloride 100 mM; sodium phosphate 20 mM; EDTA 1 mM; beta-mercaptoethanol 1 mM; glycerol 10 v/v; SDS 0.4 w/v; sodium azide 0.02 w/v; OEP16, [U-99% 13C; U-99% 15N; U-80% 2H], 1.0 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.14 M; pH: 6.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1

Software:

SPARKY v3.113, Goddard - chemical shift assignment, chemical shift calculation, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

EMBL CAA97910
SP Q41050
AlphaFold Q41050

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks