BMRB Entry 19252

Name: RRM domain of HUMAN RBM7
Published: 2015-01-26

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PDB ID: 2m8h
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19252
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

RRM domain of HUMAN RBM7

Deposition date:

2013-05-21

Original release date:

2015-01-26

Authors:

Sikorsky, Tomas; Hrossova, Dominika; Vanacova, Stepanka; Stefl, Richard

Citation:

Citation: Sikorsky, Tomas; Stefl, Richard; Hrossova, Dominika; Vanacova, Stepanka. "RNA binding properties of the human NEXT complex" .

Assembly members:

Assembly members:
RBM7, polymer, 101 residues, 11473.301 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28-SMT3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RBM7: GSEFELRRQQALAEADRTLF VGNLETKVTEELLFELFHQA GPVIKVKIPKDKDGKPKQFA FVNFKHEVSVPYAMNLLNGI KLYGRPIKIQFRSGSSHAPQ D

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	298
15N chemical shifts	80
1H chemical shifts	602

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RRM domain of HUMAN RBM7	1

Entities:

Entity 1, RRM domain of HUMAN RBM7 101 residues - 11473.301 Da.

1

GLY

SER

GLU

PHE

GLU

LEU

ARG

GLN

2

ALA

LEU

ALA

GLU

ALA

ASP

ARG

THR

LEU

PHE

3

VAL

GLY

ASN

LEU

GLU

THR

LYS

VAL

THR

GLU

4

GLU

LEU

PHE

GLU

LEU

PHE

HIS

GLN

ALA

5

GLY

PRO

VAL

ILE

LYS

VAL

LYS

ILE

PRO

LYS

6

ASP

LYS

ASP

GLY

LYS

PRO

LYS

GLN

PHE

ALA

7

PHE

VAL

ASN

PHE

LYS

HIS

GLU

VAL

SER

VAL

8

PRO

TYR

ALA

MET

ASN

LEU

ASN

GLY

ILE

9

LYS

LEU

TYR

GLY

ARG

PRO

ILE

LYS

ILE

GLN

10

PHE

ARG

SER

GLY

SER

HIS

ALA

PRO

GLN

11

ASP

Samples:

sample_1: RBM7, [U-99% 13C; U-99% 15N], 0.3 mM; BISTRIS 20 mM; beta-mercaptoethanol 4 mM; sodium chloride 200 mM

sample_conditions_1: ionic strength: 200 mM; pH: 7; pressure: 1 atm; temperature: 293.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 950 MHz

PDB	2M8H
DBJ	BAA92036 BAD92990 BAG35505 BAG73694
EMBL	CAH93091
GB	AAD39257 AAH34381 AAH70262 ADQ31580 AIC50533
REF	NP_001248000 NP_001272974 NP_057174 XP_002754472 XP_002822554
SP	Q9Y580
AlphaFold	Q9Y580