BMRB Entry 19251

Name: Structure, function, and tethering of DNA-binding domains in 54 transcriptional activators
Published: 2013-08-26

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PDB ID: 2m8g
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19251
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure, function, and tethering of DNA-binding domains in 54 transcriptional activators

Deposition date:

2013-05-19

Original release date:

2013-08-26

Authors:

HONG, EUNMI; Wemmer, David E

Citation:

Citation: Vidangos, Natasha; Maris, Ann; Young, Anisa; Hong, Eunmi; Pelton, Jeffrey; Batchelor, Joseph; Wemmer, David. "Structure, function, and tethering of DNA-binding domains in (54) transcriptional activators." Biopolymers 99, 1082-1096 (2013).
PubMed: 23818155

Assembly members:

Assembly members:
entity, polymer, 70 residues, 8334.906 Da.

Natural source:

Natural source: Common Name: aquificales Taxonomy ID: 63363 Superkingdom: Bacteria Kingdom: not available Genus/species: Aquifex aeolicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Rosetta.pLysS

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: TSSELPELLRKRERKTGDLP KFIEETEKKRIIEALEKTGY VKSRAAKLLGYTLRQLDYRI KKYGIELKKF

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	187
15N chemical shifts	58
1H chemical shifts	413

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Nlh2	1

Entities:

Entity 1, Nlh2 70 residues - 8334.906 Da.

1	THR	SER	SER	GLU	LEU	PRO	GLU	LEU	LEU	ARG
2	LYS	ARG	GLU	ARG	LYS	THR	GLY	ASP	LEU	PRO
3	LYS	PHE	ILE	GLU	GLU	THR	GLU	LYS	LYS	ARG
4	ILE	ILE	GLU	ALA	LEU	GLU	LYS	THR	GLY	TYR
5	VAL	LYS	SER	ARG	ALA	ALA	LYS	LEU	LEU	GLY
6	TYR	THR	LEU	ARG	GLN	LEU	ASP	TYR	ARG	ILE
7	LYS	LYS	TYR	GLY	ILE	GLU	LEU	LYS	LYS	PHE

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1

PDB	2M8G
GB	AAC07620
REF	NP_214227 WP_010881164

BMRB Entry 19251

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	THR	SER	SER	GLU	LEU	PRO	GLU	LEU	LEU	ARG
2	LYS	ARG	GLU	ARG	LYS	THR	GLY	ASP	LEU	PRO
3	LYS	PHE	ILE	GLU	GLU	THR	GLU	LYS	LYS	ARG
4	ILE	ILE	GLU	ALA	LEU	GLU	LYS	THR	GLY	TYR
5	VAL	LYS	SER	ARG	ALA	ALA	LYS	LEU	LEU	GLY
6	TYR	THR	LEU	ARG	GLN	LEU	ASP	TYR	ARG	ILE
7	LYS	LYS	TYR	GLY	ILE	GLU	LEU	LYS	LYS	PHE

1	THR	SER	SER	GLU	LEU	PRO	GLU	LEU	LEU	ARG
2	LYS	ARG	GLU	ARG	LYS	THR	GLY	ASP	LEU	PRO
3	LYS	PHE	ILE	GLU	GLU	THR	GLU	LYS	LYS	ARG
4	ILE	ILE	GLU	ALA	LEU	GLU	LYS	THR	GLY	TYR
5	VAL	LYS	SER	ARG	ALA	ALA	LYS	LEU	LEU	GLY
6	TYR	THR	LEU	ARG	GLN	LEU	ASP	TYR	ARG	ILE
7	LYS	LYS	TYR	GLY	ILE	GLU	LEU	LYS	LYS	PHE

1	THR	SER	SER	GLU	LEU	PRO	GLU	LEU	LEU	ARG
2	LYS	ARG	GLU	ARG	LYS	THR	GLY	ASP	LEU	PRO
3	LYS	PHE	ILE	GLU	GLU	THR	GLU	LYS	LYS	ARG
4	ILE	ILE	GLU	ALA	LEU	GLU	LYS	THR	GLY	TYR
5	VAL	LYS	SER	ARG	ALA	ALA	LYS	LEU	LEU	GLY
6	TYR	THR	LEU	ARG	GLN	LEU	ASP	TYR	ARG	ILE
7	LYS	LYS	TYR	GLY	ILE	GLU	LEU	LYS	LYS	PHE