BMRB Entry 19246

Name: 1H, 13C and 15N resonance assignments of human GAP-43
Published: 2016-10-04

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19246

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 13C and 15N resonance assignments of human GAP-43

Deposition date:

2013-05-16

Original release date:

2016-10-04

Authors:

Coudevylle, Nicolas; Konrat, Robert; Kozminski, Wiktor; Zerko, Szymon

Citation:

Citation: Flamm, Andrea; Zerko, Szymon; Zawadzka-Kazimierczuk, Anna; Kozminski, Wiktor; Konrat, Robert; Coudevylle, Nicolas. "1H, 15N, 13C resonance assignment of human GAP-43" Biomol. NMR Assign. 10, 171-174 (2016).
PubMed: 26748655

Assembly members:

Assembly members:
GAP-43, polymer, 249 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-41b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GAP-43: MLGGMRRTKQVEKNDDDQKI EQDGIKPEDKAHKAATKIQA SFRGHITRKKLKGEKKDDVQ AAEAEANKKDEAPVADGVEK KGEGTTTAEAAPATGSKPDE PGKAGETPSEEKKGEGDAAT EQAAPQAPASSEEKAGSAET ESATKASTDNSPSSKAEDAP AKEEPKQADVPAAVTAAAAT TPAAEDAAAKATAQPPTETG ESSQAEENIEAVDETKPKES ARQDEGKEEEPEADQEHAAA LEHHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	703
15N chemical shifts	219
1H chemical shifts	1021

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GAP-43	1

Entities:

Entity 1, GAP-43 249 residues - Formula weight is not available

1

MET

LEU

GLY

MET

ARG

THR

LYS

GLN

2

VAL

GLU

LYS

ASN

ASP

GLN

LYS

ILE

3

GLU

GLN

ASP

GLY

ILE

LYS

PRO

GLU

ASP

LYS

4

ALA

HIS

LYS

ALA

THR

LYS

ILE

GLN

ALA

5

SER

PHE

ARG

GLY

HIS

ILE

THR

ARG

LYS

6

LEU

LYS

GLY

GLU

LYS

ASP

VAL

GLN

7

ALA

GLU

ALA

GLU

ALA

ASN

LYS

ASP

8

GLU

ALA

PRO

VAL

ALA

ASP

GLY

VAL

GLU

LYS

9

LYS

GLY

GLU

GLY

THR

ALA

GLU

ALA

10

ALA

PRO

ALA

THR

GLY

SER

LYS

PRO

ASP

GLU

11

PRO

GLY

LYS

ALA

GLY

GLU

THR

PRO

SER

GLU

12

GLU

LYS

GLY

GLU

GLY

ASP

ALA

THR

13

GLU

GLN

ALA

PRO

GLN

ALA

PRO

ALA

SER

14

SER

GLU

LYS

ALA

GLY

SER

ALA

GLU

THR

15

GLU

SER

ALA

THR

LYS

ALA

SER

THR

ASP

ASN

16

SER

PRO

SER

LYS

ALA

GLU

ASP

ALA

PRO

17

ALA

LYS

GLU

PRO

LYS

GLN

ALA

ASP

VAL

18

PRO

ALA

VAL

THR

ALA

THR

19

THR

PRO

ALA

GLU

ASP

ALA

LYS

20

ALA

THR

ALA

GLN

PRO

THR

GLU

THR

GLY

21

GLU

SER

GLN

ALA

GLU

ASN

ILE

GLU

22

ALA

VAL

ASP

GLU

THR

LYS

PRO

LYS

GLU

SER

23

ALA

ARG

GLN

ASP

GLU

GLY

LYS

GLU

24

PRO

GLU

ALA

ASP

GLN

GLU

HIS

ALA

25

LEU

GLU

HIS

Samples:

sample_1: GAP-43, [U-95% 13C; U-95% 15N], 9.5 mM; Bis-Tris 20 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 120 mM; pH: 6; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
5D HN(CA)CONH	sample_1	isotropic	sample_conditions_1
5D (HACA)CON(CA)CONH	sample_1	isotropic	sample_conditions_1
5D (H)NCO(NCA)CONH	sample_1	isotropic	sample_conditions_1
5D HNCOCACB	sample_1	isotropic	sample_conditions_1
5D HabCabCONH	sample_1	isotropic	sample_conditions_1
5D H(CC-tocsy)CONH	sample_1	isotropic	sample_conditions_1

Software:

MFT, Wiktor Kozminski - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian DirectDrive 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks