BMRB Entry 19219

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19219
MolProbity Validation Chart

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Title:
1H, 13C, and 15N Chemical Shift Assignments for a proteasome related subunit
Deposition date:
2013-05-03
Original release date:
2015-02-02
Authors:
Wu, Yujie; Hu, Yunfei; Jin, Changwen
Citation:

Citation: Wu, Yujie; Hu, Yunfei; Jin, Changwen. "Solution Structure of Yeast Rpn9: Insights for Proteasome Lid Assembly"  J. Biol. Chem. ., .-. (2015).
PubMed: 25631053

Assembly members:

Assembly members:
a_proteasome_subunit, polymer, 401 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
a_proteasome_subunit: MFNNHEIDTILSTLRMEADP SLHPLFEQFEKFYEEKLWFQ LSESLTKFFDDAKSTPLRLR LYDNFVSKFYDKINQLSVVK YLLASLKDSKDFDESLKYLD DLKAQFQELDSKKQRNNGSK DHGDGILLIDSEIARTYLLK NDLVKARDLLDDLEKTLDKK DSIPLRITNSFYSTNSQYFK FKNDFNSFYYTSLLYLSTLE PSTSITLAERQQLAYDLSIS ALLGDKIYNFGELLHHPIME TIVNDSNYDWLFQLLNALTV GDFDKFDSLIKVQISKIPIL AQHESFLRQKICLMTLIETV FVKNIRMLSFEDISKATHLP KDNVEHLVMRAISLGLLKGS IDQVNELVTISWVQPRIISG DQITKMKDRLVEWNDQVEKL GKKMEARGQSIWVLEHHHHH H

Data sets:
Data typeCount
13C chemical shifts1513
15N chemical shifts396
1H chemical shifts2256

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Yeast Rpn91

Entities:

Entity 1, Yeast Rpn9 401 residues - Formula weight is not available

1   METPHEASNASNHISGLUILEASPTHRILE
2   LEUSERTHRLEUARGMETGLUALAASPPRO
3   SERLEUHISPROLEUPHEGLUGLNPHEGLU
4   LYSPHETYRGLUGLULYSLEUTRPPHEGLN
5   LEUSERGLUSERLEUTHRLYSPHEPHEASP
6   ASPALALYSSERTHRPROLEUARGLEUARG
7   LEUTYRASPASNPHEVALSERLYSPHETYR
8   ASPLYSILEASNGLNLEUSERVALVALLYS
9   TYRLEULEUALASERLEULYSASPSERLYS
10   ASPPHEASPGLUSERLEULYSTYRLEUASP
11   ASPLEULYSALAGLNPHEGLNGLULEUASP
12   SERLYSLYSGLNARGASNASNGLYSERLYS
13   ASPHISGLYASPGLYILELEULEUILEASP
14   SERGLUILEALAARGTHRTYRLEULEULYS
15   ASNASPLEUVALLYSALAARGASPLEULEU
16   ASPASPLEUGLULYSTHRLEUASPLYSLYS
17   ASPSERILEPROLEUARGILETHRASNSER
18   PHETYRSERTHRASNSERGLNTYRPHELYS
19   PHELYSASNASPPHEASNSERPHETYRTYR
20   THRSERLEULEUTYRLEUSERTHRLEUGLU
21   PROSERTHRSERILETHRLEUALAGLUARG
22   GLNGLNLEUALATYRASPLEUSERILESER
23   ALALEULEUGLYASPLYSILETYRASNPHE
24   GLYGLULEULEUHISHISPROILEMETGLU
25   THRILEVALASNASPSERASNTYRASPTRP
26   LEUPHEGLNLEULEUASNALALEUTHRVAL
27   GLYASPPHEASPLYSPHEASPSERLEUILE
28   LYSVALGLNILESERLYSILEPROILELEU
29   ALAGLNHISGLUSERPHELEUARGGLNLYS
30   ILECYSLEUMETTHRLEUILEGLUTHRVAL
31   PHEVALLYSASNILEARGMETLEUSERPHE
32   GLUASPILESERLYSALATHRHISLEUPRO
33   LYSASPASNVALGLUHISLEUVALMETARG
34   ALAILESERLEUGLYLEULEULYSGLYSER
35   ILEASPGLNVALASNGLULEUVALTHRILE
36   SERTRPVALGLNPROARGILEILESERGLY
37   ASPGLNILETHRLYSMETLYSASPARGLEU
38   VALGLUTRPASNASPGLNVALGLULYSLEU
39   GLYLYSLYSMETGLUALAARGGLYGLNSER
40   ILETRPVALLEUGLUHISHISHISHISHIS
41   HIS

Samples:

sample_1: protein, [U-100% 13C; U-100% 15N], 0.7 mM; DTT 10 mM; MES 50 mM; DSS 0.03%; H2O 90%; D2O 10%

sample_2: protein, [U-13C; U-15N; U-2H], 0.7 mM; DTT 10 mM; MES 50 mM; DSS 0.03%; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HN(COCA)CBsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 500 MHz

Related Database Links:

PDB
DBJ GAA22644
EMBL CAY78927
GB AAB64853 AHY75380 AJP38106 AJU58228 AJU58921
REF NP_010715
SP Q04062
TPG DAA12266
AlphaFold Q04062

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks