BMRB Entry 19214

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PDB ID: 2m7v
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19214
MolProbity Validation Chart

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Title:
Green Light-Absorbing State of TePixJ, an Active Cyanobacteriochrome Domain
Deposition date:
2013-05-01
Original release date:
2014-01-02
Authors:
Cornilescu, Gabriel; Cornilescu, Claudia; Burgie, Sethe; Walker, Joseph; Markley, John; Ulijasz, Andrew; Vierstra, Richard
Citation:

Citation: Cornilescu, Claudia; Cornilescu, Gabriel; Burgie, E. Sethe; Markley, John; Ulijasz, Andrew; Vierstra, Richard. "Dynamic Structural Changes Underpin Photoconversion of a Blue/Green Cyanobacteriochrome between Its Dark and Photoactivated States."  J. Biol. Chem. 289, 3055-3065 (2014).
PubMed: 24337572

Assembly members:

Assembly members:
entity_1, polymer, 165 residues, 18769.467 Da.
PHYCOCYANOBILIN, non-polymer, 588.694 Da.

Natural source:

Natural source:   Common Name: Thermosynechococcus elongatus   Taxonomy ID: 146786   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermosynechococcus elongatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pBAD-Myc-His

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAAVQLSELRDRQAIFETLV AKGRELLACDRVIVYAFDDN YVGTVVAESVAEGWPQARDQ VIEDPCFREHWVEAYRQGRI QATTDIFKAGLTECHLNQLR PLKVRANLVVPMVIDDQLFG LLIAHQASEPRQWQEIEIDQ FSELASTGSLVLERLHFLEQ TIASL

Data sets:
Data typeCount
13C chemical shifts547
15N chemical shifts145
1H chemical shifts852

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1TePixJ1
2PHYCOCYANOBILIN2

Entities:

Entity 1, TePixJ 165 residues - 18769.467 Da.

1   METALAALAVALGLNLEUSERGLULEUARG
2   ASPARGGLNALAILEPHEGLUTHRLEUVAL
3   ALALYSGLYARGGLULEULEUALACYSASP
4   ARGVALILEVALTYRALAPHEASPASPASN
5   TYRVALGLYTHRVALVALALAGLUSERVAL
6   ALAGLUGLYTRPPROGLNALAARGASPGLN
7   VALILEGLUASPPROCYSPHEARGGLUHIS
8   TRPVALGLUALATYRARGGLNGLYARGILE
9   GLNALATHRTHRASPILEPHELYSALAGLY
10   LEUTHRGLUCYSHISLEUASNGLNLEUARG
11   PROLEULYSVALARGALAASNLEUVALVAL
12   PROMETVALILEASPASPGLNLEUPHEGLY
13   LEULEUILEALAHISGLNALASERGLUPRO
14   ARGGLNTRPGLNGLUILEGLUILEASPGLN
15   PHESERGLULEUALASERTHRGLYSERLEU
16   VALLEUGLUARGLEUHISPHELEUGLUGLN
17   THRILEALASERLEU

Entity 2, PHYCOCYANOBILIN - C33 H40 N4 O6 - 588.694 Da.

1   CYC

Related Database Links:

BMRB 19213
PDB 2M7U 2M7V 3VV4 4FOF 4GLQ
DBJ BAC08121
REF NP_681359 WP_011056417

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