BMRB Entry 19201

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19201
MolProbity Validation Chart

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NMR-STAR v3 text file.
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Title:
TAX1BP1 UBZ1+2
Deposition date:
2013-04-29
Original release date:
2013-12-02
Authors:
Ceregido, M. Angeles; Spinola, Mercedes; Buts, Lieven; Bravo, Jeronimo; van Nuland, Nico
Citation:

Citation: Ceregido, M. Angeles; Spinola Amilibia, Mercedes; Buts, Lieven; Rivera-Torres, Jose; Garcia-Pino, Abel; Bravo, Jeronimo; van Nuland, Nico. "The Structure of TAX1BP1 UBZ1+2 Provides Insight into Target Specificity and Adaptability."  J. Mol. Biol. 426, 674-690 (2014).
PubMed: 24239949

Assembly members:

Assembly members:
TAX1BP1_UBZ1+2, polymer, 69 residues, 8332.372 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TAX1BP1_UBZ1+2: GPHMDVHKKCPLCELMFPPN YDQSKFEEHVESHWKVCPMC SEQFPPDYDQQVFERHVQTH FDQNVLNFD

Data sets:
Data typeCount
13C chemical shifts295
15N chemical shifts72
1H chemical shifts466

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TAX1BP1_UBZ1+21
2ZINC ION_12
3ZINC ION_22

Entities:

Entity 1, TAX1BP1_UBZ1+2 69 residues - 8332.372 Da.

1   GLYPROHISMETASPVALHISLYSLYSCYS
2   PROLEUCYSGLULEUMETPHEPROPROASN
3   TYRASPGLNSERLYSPHEGLUGLUHISVAL
4   GLUSERHISTRPLYSVALCYSPROMETCYS
5   SERGLUGLNPHEPROPROASPTYRASPGLN
6   GLNVALPHEGLUARGHISVALGLNTHRHIS
7   PHEASPGLNASNVALLEUASNPHEASP

Entity 2, ZINC ION_1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: UBZ1+2, [U-98% 13C; U-98% 15N], 0.5 – 1.0 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
CCH-TOCSYsample_1isotropicsample_conditions_1
(HB)CB(CGCD)HDsample_1isotropicsample_conditions_1
(HB)CB(CGCDCE)HEsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR, CCPN - chemical shift assignment, data analysis, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Varian VNS 600 MHz
  • Varian VNS 800 MHz

Related Database Links:

PDB
DBJ BAB23383 BAE26880 BAG36949 BAG59309 BAG61627
EMBL CAB66521 CAG33337 CAG38587 CAH93100 CAH93223
GB AAA75595 AAC13359 AAF24016 AAG03025 AAH50358
REF NP_001004199 NP_001073333 NP_001126895 NP_001128892 NP_001193830
SP Q3UKC1 Q5R4U3 Q66HA4 Q86VP1
AlphaFold Q3UKC1 Q5R4U3 Q66HA4 Q86VP1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks