BMRB Entry 19187

Name: 1H, 13C, and 15N Chemical Shift Assignments for the C-terminus of the minichromosome maintenance protein MCM from Methanothermobacter thermautotrophicus
Published: 2013-09-30

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PDB ID: 2ma3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19187
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for the C-terminus of the minichromosome maintenance protein MCM from Methanothermobacter thermautotrophicus

Deposition date:

2013-04-24

Original release date:

2013-09-30

Authors:

Wiedemann, Christoph; Ohlenschlager, Oliver; Medagli, Barbara; Onesti, Silvia; Gorlach, Matthias

Citation:

Citation: Wiedemann, Christoph; Ohlenschlager, Oliver; Medagli, Barbara; Onesti, Silvia; Gorlach, Matthias. "(1)H, (15)N and (13)C chemical shift assignments for the winged helix domains of two archeal MCM C-termini." Biomol. NMR Assignments ., .-. (2013).
PubMed: 23934138

Assembly members:

Assembly members:
C-terminus_MCM, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Methanothermobacter thermautotrophicus Taxonomy ID: 145262 Superkingdom: Archaea Kingdom: not available Genus/species: Methanothermobacter thermautotrophicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pPROEX HTa

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-terminus_MCM: GAMGETGKIDIDKVEGRTPK SERDKFRLLLELIKEYEDDY GGRAPTNILITEMMDRYNVS EEKVEELIRILKDKGAIFEP ARGYLKIV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	405
15N chemical shifts	83
1H chemical shifts	645

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Methanothermobacter thermautotrophicus MCM C-terminus	1

Entities:

Entity 1, Methanothermobacter thermautotrophicus MCM C-terminus 88 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

GLU

THR

GLY

LYS

ILE

ASP

2

ILE

ASP

LYS

VAL

GLU

GLY

ARG

THR

PRO

LYS

3

SER

GLU

ARG

ASP

LYS

PHE

ARG

LEU

4

GLU

LEU

ILE

LYS

GLU

TYR

GLU

ASP

TYR

5

GLY

ARG

ALA

PRO

THR

ASN

ILE

LEU

ILE

6

THR

GLU

MET

ASP

ARG

TYR

ASN

VAL

SER

7

GLU

LYS

VAL

GLU

LEU

ILE

ARG

ILE

8

LEU

LYS

ASP

LYS

GLY

ALA

ILE

PHE

GLU

PRO

9

ALA

ARG

GLY

TYR

LEU

LYS

ILE

VAL

Samples:

sample_1: C-terminus of the minichromosome maintenance protein MCM of Methanothermobacter thermautotrophicus, [U-13C; U-15N], 1 mM; sodium phosphate 10 mM; sodium chloride 150 mM; H20 90%; D2O 10%

sample_2: C-terminus of the minichromosome maintenance protein MCM of Methanothermobacter thermautotrophicus, [U-13C; U-15N], 1 mM; sodium phosphate 10 mM; sodium chloride 150 mM; D2O 100%

sample_conditions_1: ionic strength: 160 mM; pH: 6.2; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CcpNMR, CCPN - chemical shift assignment, data analysis

NMR spectrometers:

Bruker AvanceIII 750 MHz

UniProtKB	O27798
PDB	2MA3
GB	AAB86236
REF	WP_048061327