BMRB Entry 19181

Name: 1H, and 15N Chemical Shift Assignments for mycoloylated PorH in DMSO solution
Published: 2013-09-25

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19181

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, and 15N Chemical Shift Assignments for mycoloylated PorH in DMSO solution

Deposition date:

2013-04-19

Original release date:

2013-09-25

Authors:

Saurel, Olivier; Demange, Pascal; Rath, Parth; Tropis, Maryelle; Daffe, Mamadou; MILON, Alain

Citation:

Citation: Rath, Parthasarathi; Saurel, Olivier; Tropis, Maryelle; Daffe, Mamadou; Demange, Pascal; Milon, Alain. "NMR localization of the O-mycoloylation on PorH, a channel forming peptide from Corynebacterium glutamicum." FEBS Lett. 587, 3687-3691 (2013).
PubMed: 24100136

Assembly members:

Assembly members:
PorH, polymer, 74 residues, Formula weight is not available

Natural source:

Natural source: Common Name: High GC Gram+ Taxonomy ID: 1718 Superkingdom: Bacteria Kingdom: not available Genus/species: corynebacterium glutamicum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: C. glutamicum PorA PorH Vector: pXMJ19-PorHCHis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PorH: MDLSLLKETLGNYETFGGNI GTALQSIPTLLDSILNFFDN FGDLADTTGENLDNFSSIEG RASRGSHHHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	66
1H chemical shifts	351

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PorH	1

Entities:

Entity 1, PorH 74 residues - Formula weight is not available

1

MET

ASP

LEU

SER

LEU

LYS

GLU

THR

LEU

2

GLY

ASN

TYR

GLU

THR

PHE

GLY

ASN

ILE

3

GLY

THR

ALA

LEU

GLN

SER

ILE

PRO

THR

LEU

4

LEU

ASP

SER

ILE

LEU

ASN

PHE

ASP

ASN

5

PHE

GLY

ASP

LEU

ALA

ASP

THR

GLY

GLU

6

ASN

LEU

ASP

ASN

PHE

SER

ILE

GLU

GLY

7

ARG

ALA

SER

ARG

GLY

SER

HIS

8

HIS

Samples:

sample_1: PorH, [U-99% 15N], 1 mM; mycolic acid 1 mM; DMSO 100%

sample_conditions_1: temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

EMBL	CAF20738 CAI39424
GB	AGT06429