BMRB Entry 19156

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19156

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Title:
1H, 13C, and 15N NMR Assignments of a Hedgehog Autoprocessing Domain
Deposition date:
2013-04-10
Original release date:
2014-02-12
Authors:
Xie, Jian; Du, Zhenming; Callahan, Brian; Wang, Chunyu
Citation:

Citation: Xie, Jian; Du, Zhenming; Callahan, Brian; Belfort, Marlene; Wang, Chunyu. "(1)H, (13)C, and (15)N NMR assignments of a Drosophila Hedgehog autoprocessing domain."  Biomol. NMR Assignments ., .-. (2013).
PubMed: 23765287

Assembly members:

Assembly members:
Hedgehog_Autoprocessing_Domain, polymer, 146 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-45b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hedgehog_Autoprocessing_Domain: CFTPESTALLESGVRKPLGE LSIGDRVLSMTANGQAVYSE VILFMDRNLEQMQNFVQLHT DGGAVLTVTPAHLVSVWQPE SQKLTFVFADRIEEKNQVLV RDVETGELRPQRVVKVGSVR SKGVVAPLTREGTIVVNSVA ASCYAV

Data sets:
Data typeCount
13C chemical shifts581
15N chemical shifts265
1H chemical shifts1077

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hedgehog Autoprocessing Domain1

Entities:

Entity 1, Hedgehog Autoprocessing Domain 146 residues - Formula weight is not available

1   CYSPHETHRPROGLUSERTHRALALEULEU
2   GLUSERGLYVALARGLYSPROLEUGLYGLU
3   LEUSERILEGLYASPARGVALLEUSERMET
4   THRALAASNGLYGLNALAVALTYRSERGLU
5   VALILELEUPHEMETASPARGASNLEUGLU
6   GLNMETGLNASNPHEVALGLNLEUHISTHR
7   ASPGLYGLYALAVALLEUTHRVALTHRPRO
8   ALAHISLEUVALSERVALTRPGLNPROGLU
9   SERGLNLYSLEUTHRPHEVALPHEALAASP
10   ARGILEGLUGLULYSASNGLNVALLEUVAL
11   ARGASPVALGLUTHRGLYGLULEUARGPRO
12   GLNARGVALVALLYSVALGLYSERVALARG
13   SERLYSGLYVALVALALAPROLEUTHRARG
14   GLUGLYTHRILEVALVALASNSERVALALA
15   ALASERCYSTYRALAVAL

Samples:

sample_1: sodium phosphate 50 mM; DTT 10 mM; sodium chloride 100 mM; Hedgehog Autoprocessing Domain, [U-98% 15N], 0.7 mM; H2O 90%; D2O 10%

sample_2: sodium phosphate 50 mM; DTT 10 mM; sodium chloride 100 mM; Hedgehog Autoprocessing Domain, [U-98% 13C; U-98% 15N], 0.6 mM; H2O 90%; D2O 10%

sample_3: sodium phosphate 50 mM; DTT 10 mM; sodium chloride 100 mM; Hedgehog Autoprocessing Domain, [U-98% 13C; U-98% 15N], 0.6 mM; H2O 90%; D2O 10%

sample_4: sodium phosphate 50 mM; DTT 10 mM; sodium chloride 100 mM; Hedgehog Autoprocessing Domain, [U-98% 13C; U-98% 15N; U-90% 2H], 0.6 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K

sample_conditions_2: pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_4isotropicsample_conditions_2
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCACBsample_4isotropicsample_conditions_2
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CACBsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCACOsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
3D H(CC)(CO)NHsample_2isotropicsample_conditions_1
3D (H)C(CCO)NHsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
GB AAA16458 AAA28604 AAB28646 ABC66186 EDV54047
PRF 1905316A
REF NP_001034065 XP_001982177 XP_002032309 XP_002098392
SP B3P7F8 B4HFB7 B4PN49 Q02936
AlphaFold B3P7F8 B4HFB7 B4PN49 Q02936

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks