BMRB Entry 19154
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR19154
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Citation: Um, Soohyun; Kim, Young-Joo; Kwon, Hyuknam; Wen, He; Kim, Seong-Hwan; Kwon, Hak Cheol; Park, Sunghyouk; Shin, Jongheon; Oh, Dong-Chan. "Sungsanpin, a lasso Peptide from a deep-sea streptomycete." J. Nat. Prod. 76, 873-879 (2013).
PubMed: 23662937
Assembly members:
Sungsanpin, polymer, 15 residues, 1591 Da.
Natural source: Common Name: Streptomyces Taxonomy ID: 742659 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptomyces not available
Experimental source: Production method: purified from the natural source Host organism: Streptomyces sp. I08A-00426
Entity Sequences (FASTA):
Sungsanpin: GFGSKPIDSFGLSWL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 77 |
| 1H chemical shifts | 103 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sungsanpin | 1 |
Entities:
Entity 1, Sungsanpin 15 residues - 1591 Da.
| 1 | GLY | PHE | GLY | SER | LYS | PRO | ILE | ASP | SER | PHE | ||||
| 2 | GLY | LEU | SER | TRP | LEU |
Samples:
sample_1: Sungsanpin 18.2 mg; pyridine-d5
sample_conditions_1: pH: 5.25; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Bruker ARX 900 MHz