BMRB Entry 19147

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PDB ID: 2m6n
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19147
MolProbity Validation Chart

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Title:
ZINC Binding Domain
Deposition date:
2013-04-06
Original release date:
2014-01-24
Authors:
Frye, Jeremiah; Brown, Nicholas; Petzold, Georg; Watson, Edmond; Royappa, Grace; Nourse, Amanda; Jarvis, Marc; Kriwacki, Richard; Peters, Jan-Michael; Stark, Holger; Schulman, Brenda
Citation:

Citation: Frye, Jeremiah; Brown, Nicholas; Petzold, Georg; Watson, Edmond; Royappa, Grace; Nourse, Amanda; Jarvis, Marc; Kriwacki, Richard; Peters, Jan-Michael; Stark, Holger; Schulman, Brenda. "EM Structure of APC/C_CDH1-EMI1: multimodal mechanism of E3 ligase shutdown"  Nat. Struct. Biol. ., .-..

Assembly members:

Assembly members:
APC/C_CDH1-EMI1, polymer, 86 residues, 9605.396 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX4T1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
APC/C_CDH1-EMI1: GSEVAKTLKKNESLKACIRC NSPAKYDCYLQRATCKREGC GFDYCTKCLCNYHTTKDCSD GKLLKASCKIGPLPGTKKSK KNLRRL

Data sets:
Data typeCount
13C chemical shifts355
15N chemical shifts86
1H chemical shifts601

Additional metadata:

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  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1APC/C_CDH1-EMI11
2ZINC ION_12
3ZINC ION_22

Entities:

Entity 1, APC/C_CDH1-EMI1 86 residues - 9605.396 Da.

1   GLYSERGLUVALALALYSTHRLEULYSLYS
2   ASNGLUSERLEULYSALACYSILEARGCYS
3   ASNSERPROALALYSTYRASPCYSTYRLEU
4   GLNARGALATHRCYSLYSARGGLUGLYCYS
5   GLYPHEASPTYRCYSTHRLYSCYSLEUCYS
6   ASNTYRHISTHRTHRLYSASPCYSSERASP
7   GLYLYSLEULEULYSALASERCYSLYSILE
8   GLYPROLEUPROGLYTHRLYSLYSSERLYS
9   LYSASNLEUARGARGLEU

Entity 2, ZINC ION_1 - Zn - 65.409 Da.

1   ZN

Related Database Links:

PDB 2M6N 4UI9
DBJ BAG37064 BAG51487
GB AAF04469 AAH18905 AAL86610 ABM82848 ABM86032
REF NP_001135994 NP_036309 XP_001096104 XP_001141083 XP_002747165
SP Q9UKT4
AlphaFold Q9UKT4

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