BMRB Entry 19137

Name: ERGi Backbone Chemical Shifts
Published: 2013-08-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19137

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

ERGi Backbone Chemical Shifts

Deposition date:

2013-04-02

Original release date:

2013-08-15

Authors:

Regan, Michael

Citation:

Citation: Regan, Michael; Horanyi, Peter; Pryor, Edward; Sarver, Jessica; Cafiso, David; Bushweller, John. "Structural and dynamic studies of the transcription factor ERG reveal DNA binding is allosterically autoinhibited." Proc. Natl. Acad. Sci. U.S.A. 110, 13374-13379 (2013).
PubMed: 23898196

Assembly members:

Assembly members:
ERGi, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHis-Parallel2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ERGi: GAMAPQLDPYQILGPTSSRL ANPGSGQIQLWQFLLELLSD SSNSSCITWEGTNGEFKMTD PDEVARRWGERKSKPNMNYD KLSRALRYYYDKNIMTKVHG KRYAYKFDFHGIAQALQPHP PE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	280
15N chemical shifts	88
1H chemical shifts	175

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ERGi	1

Entities:

Entity 1, ERGi 122 residues - Formula weight is not available

1

GLY

ALA

MET

ALA

PRO

GLN

LEU

ASP

PRO

TYR

2

GLN

ILE

LEU

GLY

PRO

THR

SER

ARG

LEU

3

ALA

ASN

PRO

GLY

SER

GLY

GLN

ILE

GLN

LEU

4

TRP

GLN

PHE

LEU

GLU

LEU

SER

ASP

5

SER

ASN

SER

CYS

ILE

THR

TRP

GLU

6

GLY

THR

ASN

GLY

GLU

PHE

LYS

MET

THR

ASP

7

PRO

ASP

GLU

VAL

ALA

ARG

TRP

GLY

GLU

8

ARG

LYS

SER

LYS

PRO

ASN

MET

ASN

TYR

ASP

9

LYS

LEU

SER

ARG

ALA

LEU

ARG

TYR

10

ASP

LYS

ASN

ILE

MET

THR

LYS

VAL

HIS

GLY

11

LYS

ARG

TYR

ALA

TYR

LYS

PHE

ASP

PHE

HIS

12

GLY

ILE

ALA

GLN

ALA

LEU

GLN

PRO

HIS

PRO

13

PRO

GLU

Samples:

sample_1: ERGi, [U-98% 13C; U-98% 15N], 0.5 mM; H2O 95%; D2O 5%; MgSO4 0.2 M; KPI 0.02 M; NaN3 0.01%; DTT 0.005 M

sample_conditions_1: ionic strength: 0.2 M; pH: 6.0; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

BMRB	19136 19138
PDB	1FLI 4IRH 4IRI
DBJ	BAB62744 BAB69948 BAB69949 BAB69950 BAC34461
EMBL	CAA47389 CAA54404 CAA75077 CAA75078 CAB46566
GB	AAA35811 AAA52398 AAB28525 AAB31417 AAB31419
REF	NP_001008616 NP_001026079 NP_001079309 NP_001079310 NP_001084371
SP	P11308 P41157 P81270 Q90837
TPG	DAA32951
AlphaFold	P11308 P41157 P81270 Q90837