BMRB Entry 19135

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19135

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Title:
Efficient Protocol for Assignment of Large, Intrinsically Disordered Proteins. 49.2kDa MAP2c, a case study
Deposition date:
2013-04-02
Original release date:
2013-08-15
Authors:
Novacek, Jiri; Janda, Lubomir; Dopitova, Radka; Sklenar, Vladimir; Zidek, Lukas
Citation:

Citation: Novaek, Jii; Janda, Lubomir; Dopitova, Radka; idek, Luka; Sklena, Vladimir. "Efficient protocol for backbone and side-chain assignments of large, intrinsically disordered proteins: transient secondary structure analysis of 49.2 kDa microtubule associated protein 2c."  J. Biomol. NMR 56, 291-301 (2013).
PubMed: 23877929

Assembly members:

Assembly members:
MAP2c, polymer, 467 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MAP2c: MADERKDEGKAPHWTSASLT EAAAHPHSPEMKDQGGSGEG LSRSANGFPYREEEEGAFGE HGSQGTYSDTKENGINGELT SADRETAEEVSARIVQVVTA EAVAVLKGEQEKEAQHKDQP AALPLAAEETVNLPPSPPPS PASEQTAALEEATSGESAQA PSAFKQAKDKVTDGITKSPE KRSSLPRPSSILPPRRGVSG DREENSFSLNSSISSARRTT RSEPIRRAGKSGTSTPTTPG STAITPGTPPSYSSRTPGTP GTPSYPRTPGTPKSGILVPS EKKVAIIRTPPKSPATPKQL RLINQPLPDLKNVKSKIGST DNIKYQPKGGQVQIVTKKID LSHVTSKCGSLKNIRHRPGG GRVKIESVKLDFKEKAQAKV GSLDNAHHVPGGGNVKIDSQ KLNFREHAKARVDHGAEIIT QSPSRSSVASPRRLSNVSSS GSINLLESPQLATLAEDVTA ALAKQGL

Data sets:
Data typeCount
13C chemical shifts1847
15N chemical shifts465
1H chemical shifts2363

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MAP2c1

Entities:

Entity 1, MAP2c 467 residues - Formula weight is not available

1   METALAASPGLUARGLYSASPGLUGLYLYS
2   ALAPROHISTRPTHRSERALASERLEUTHR
3   GLUALAALAALAHISPROHISSERPROGLU
4   METLYSASPGLNGLYGLYSERGLYGLUGLY
5   LEUSERARGSERALAASNGLYPHEPROTYR
6   ARGGLUGLUGLUGLUGLYALAPHEGLYGLU
7   HISGLYSERGLNGLYTHRTYRSERASPTHR
8   LYSGLUASNGLYILEASNGLYGLULEUTHR
9   SERALAASPARGGLUTHRALAGLUGLUVAL
10   SERALAARGILEVALGLNVALVALTHRALA
11   GLUALAVALALAVALLEULYSGLYGLUGLN
12   GLULYSGLUALAGLNHISLYSASPGLNPRO
13   ALAALALEUPROLEUALAALAGLUGLUTHR
14   VALASNLEUPROPROSERPROPROPROSER
15   PROALASERGLUGLNTHRALAALALEUGLU
16   GLUALATHRSERGLYGLUSERALAGLNALA
17   PROSERALAPHELYSGLNALALYSASPLYS
18   VALTHRASPGLYILETHRLYSSERPROGLU
19   LYSARGSERSERLEUPROARGPROSERSER
20   ILELEUPROPROARGARGGLYVALSERGLY
21   ASPARGGLUGLUASNSERPHESERLEUASN
22   SERSERILESERSERALAARGARGTHRTHR
23   ARGSERGLUPROILEARGARGALAGLYLYS
24   SERGLYTHRSERTHRPROTHRTHRPROGLY
25   SERTHRALAILETHRPROGLYTHRPROPRO
26   SERTYRSERSERARGTHRPROGLYTHRPRO
27   GLYTHRPROSERTYRPROARGTHRPROGLY
28   THRPROLYSSERGLYILELEUVALPROSER
29   GLULYSLYSVALALAILEILEARGTHRPRO
30   PROLYSSERPROALATHRPROLYSGLNLEU
31   ARGLEUILEASNGLNPROLEUPROASPLEU
32   LYSASNVALLYSSERLYSILEGLYSERTHR
33   ASPASNILELYSTYRGLNPROLYSGLYGLY
34   GLNVALGLNILEVALTHRLYSLYSILEASP
35   LEUSERHISVALTHRSERLYSCYSGLYSER
36   LEULYSASNILEARGHISARGPROGLYGLY
37   GLYARGVALLYSILEGLUSERVALLYSLEU
38   ASPPHELYSGLULYSALAGLNALALYSVAL
39   GLYSERLEUASPASNALAHISHISVALPRO
40   GLYGLYGLYASNVALLYSILEASPSERGLN
41   LYSLEUASNPHEARGGLUHISALALYSALA
42   ARGVALASPHISGLYALAGLUILEILETHR
43   GLNSERPROSERARGSERSERVALALASER
44   PROARGARGLEUSERASNVALSERSERSER
45   GLYSERILEASNLEULEUGLUSERPROGLN
46   LEUALATHRLEUALAGLUASPVALTHRALA
47   ALALEUALALYSGLNGLYLEU

Samples:

sample_1: MAP2c, [U-100% 13C; U-100% 15N], 1.2 mM; MOPS 50 mM; sodium chloride 100 mM; TCEP 0.7 mM; H2O 90%; D2O 20%

sample_conditions_1: ionic strength: 0.15 M; pH: 6.9; pressure: 1 atm; temperature: 300.15 K

Experiments:

NameSampleSample stateSample conditions
5D CACONCACOsample_1isotropicsample_conditions_1
5D HC(CC-TOCSY)CACONsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

EMBL CAA35667
GB AAB32526 AAH48781 AAH51410 AAH52446 AAI50946
PIR JC7923
REF NP_032658 XP_006495825 XP_006536975 XP_008765430 XP_011236752