BMRB Entry 19106

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19106
MolProbity Validation Chart

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Title:
The protease-resistant N-terminal domain of TIR-domain containing adaptor molecule-1, TICAM-1
Deposition date:
2013-03-20
Original release date:
2014-02-12
Authors:
Kumeta, Hiroyuki; Enokizono, Yoshiaki; Sakakibara, Hiromi; Ogura, Kenji; Matsumoto, Misako; Seya, Tsukasa; Inagaki, Fuyuhiko
Citation:

Citation: Kumeta, Hiroyuki; Sakakibara, Hiromi; Enokizono, Yoshiaki; Ogura, Kenji; Horiuchi, Masataka; Matsumoto, Misako; Seya, Tsukasa; Inagaki, Fuyuhiko. "The N-terminal domain of TIR domain-containing adaptor molecule-1, TICAM-1."  J. Biomol. NMR 58, 227-230 (2014).
PubMed: 24500696

Assembly members:

Assembly members:
TICAM-1_NTD, polymer, 159 residues, 17244.688 Da.

Natural source:

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6p

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TICAM-1_NTD: GPHMACTGPSLPSAFDILGA AGQDKLLYLKHKLKTPRPGC QGQDLLHAMVLLKLGQETEA RISLEALKADAVARLVARQW AGVDSTEDPEEPPDVSWAVA RLYHLLAEEKLCPASLRDVA YQEAVRTLSSRDDHRLGELQ DEARNRCGWDIAGDPGSIR

Data sets:
Data typeCount
13C chemical shifts692
15N chemical shifts174
1H chemical shifts1141

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TICAM-11

Entities:

Entity 1, TICAM-1 159 residues - 17244.688 Da.

1   GLYPROHISMETALACYSTHRGLYPROSER
2   LEUPROSERALAPHEASPILELEUGLYALA
3   ALAGLYGLNASPLYSLEULEUTYRLEULYS
4   HISLYSLEULYSTHRPROARGPROGLYCYS
5   GLNGLYGLNASPLEULEUHISALAMETVAL
6   LEULEULYSLEUGLYGLNGLUTHRGLUALA
7   ARGILESERLEUGLUALALEULYSALAASP
8   ALAVALALAARGLEUVALALAARGGLNTRP
9   ALAGLYVALASPSERTHRGLUASPPROGLU
10   GLUPROPROASPVALSERTRPALAVALALA
11   ARGLEUTYRHISLEULEUALAGLUGLULYS
12   LEUCYSPROALASERLEUARGASPVALALA
13   TYRGLNGLUALAVALARGTHRLEUSERSER
14   ARGASPASPHISARGLEUGLYGLULEUGLN
15   ASPGLUALAARGASNARGCYSGLYTRPASP
16   ILEALAGLYASPPROGLYSERILEARG

Samples:

sample_1: TICAM-1_NTD, [U-99% 13C; U-99% 15N], 0.75 mM; DTT 3 mM; sodium phosphate 50 mM; sodium azide 1 mM; DSS 0.02 mg/mL; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)HAsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

VNMR v6.1C -

NMR spectrometers:

  • Varian Unity INOVA 800 MHz
  • Varian Unity INOVA 600 MHz

Related Database Links:

PDB
DBJ BAC44839 BAC55579 BAC77376 BAG55261 BAG55262
GB AAI36557 AAI36558 AIC61374 EAW69193 EAW69194
REF NP_001123604 NP_001266155 NP_001266518 NP_891549 XP_002828536
SP Q8IUC6
AlphaFold Q8IUC6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks