BMRB Entry 19104

Name: Induced folding in RNA recognition by Arabidopsis thaliana DCL1
Published: 2015-09-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19104

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Induced folding in RNA recognition by Arabidopsis thaliana DCL1

Deposition date:

2013-03-19

Original release date:

2015-09-09

Authors:

Suarez, Irina; Burdisso, Paula; Benoit, Matthieu; Boisbouvier, Jerome; Rasia, Rodolfo

Citation:

Citation: Suarez, Irina; Burdisso, Paula; Benoit, Matthieu; Boisbouvier, Jerome; Rasia, Rodolfo. "Induced folding in RNA recognition by Arabidopsis thaliana DCL1" Nucleic Acids Res. 43, 6607-6619 (2015).
PubMed: 26101256

Assembly members:

Assembly members:
NB22, polymer, 83 residues, 9160.3879 Da.

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-TEV

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NB22: GHMMHPVRELQERCQQQAEG LEYKASRSGNTATVEVFIDG VQVGVAQNPQKKMAQKLAAR NALAALKEKEIAESKEKHIN NGN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list
spectral_peak_list

Data type	Count
13C chemical shifts	225
15N chemical shifts	74
1H chemical shifts	74

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NB22_1	1
2	NB22_2	1
3	NB22_3	1

Entities:

Entity 1, NB22_1 83 residues - 9160.3879 Da.

1

GLY

HIS

MET

HIS

PRO

VAL

ARG

GLU

LEU

2

GLN

GLU

ARG

CYS

GLN

ALA

GLU

GLY

3

LEU

GLU

TYR

LYS

ALA

SER

ARG

SER

GLY

ASN

4

THR

ALA

THR

VAL

GLU

VAL

PHE

ILE

ASP

GLY

5

VAL

GLN

VAL

GLY

VAL

ALA

GLN

ASN

PRO

GLN

6

LYS

MET

ALA

GLN

LYS

LEU

ALA

ARG

7

ASN

ALA

LEU

ALA

LEU

LYS

GLU

LYS

GLU

8

ILE

ALA

GLU

SER

LYS

GLU

LYS

HIS

ILE

ASN

9

ASN

GLY

ASN

Samples:

sample_1: NB22, [U-13C; U-15N; U-2H], 400 uM; miR172ls 500 uM; sodium phosphate 20 mM; TCEP 5 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 20 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC/HMQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
HNCoCACB_NB22_RNA (H[N[co[{CA\|ca[C]}]]])	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

ANALYSIS v2.1, CCPN - secuential assignment

NMR spectrometers:

Varian DirectDrive 600 MHz

BMRB	19105
DBJ	BAD94606
GB	AAF03534 AAF26461 AAG38019 AAG38020 AEE27220
REF	NP_001184881 NP_171612 XP_006306578 XP_006418369
SP	Q9SP32
AlphaFold	Q9SP32